Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings.
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ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC8567432 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Liquid culture system for efficient depletion of the endogenous nutrients in Arabidopsis seedlings.
STAR protocols 20211029 4
Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture system for efficient depletion of the endogenous carbon/nitrogen nutrients in Arabidopsis seedlings. Exogenous carbon/nitrogen can be supplied to dissect the TOR pathway. For complete details on the use and execution o ...[more]
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