Dataset Information

Characterization of the complete chloroplast genome of Rhododendron henanense subsp. lingbaoense Fang.

ABSTRACT: Rhododendron henanense subsp. lingbaoense is endemic in China. The cpDNA of R. henanense subsp. lingbaoense is a typical quadripartite structure with a length of 208,015 bp, including a large single-copy region of 110,593 bp and a small single-copy region of 2606 bp separated by a pair of identical inverted repeat regions of 47,408 bp each. The chloroplast genome contains 119 genes, including 86 protein-coding genes, four ribosomal RNA genes, and 29 transfer RNA genes. The phylogenetic analysis of R. henanense subsp. lingbaoense showed a relatively close relationship with Rhododendron delavayi.

SUBMITTER: Zhou XJ

PROVIDER: S-EPMC8567889 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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Characterization of the complete chloroplast genome of Rhododendron henanense subsp. lingbaoense Fang.

Zhou Xiao-Jun XJ Wei Meng-Jiao MJ Zhang Kai K Han Jun-Wang JW Wang Hai-Liang HL Dong Shuai-Wei SW

Mitochondrial DNA. Part B, Resources 20211103 12

Rhododendron henanense subsp. lingbaoense is endemic in China. The cpDNA of R. henanense subsp. lingbaoense is a typical quadripartite structure with a length of 208,015 bp, including a large single-copy region of 110,593 bp and a small single-copy region of 2606 bp separated by a pair of identical inverted repeat regions of 47,408 bp each. The chloroplast genome contains 119 genes, including 86 protein-coding genes, four ribosomal RNA genes, and 29 transfer RNA genes ...[more]

PMID: 34746402

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Project description:In this study, we aimed to investigate the heat tolerance mechanism in Rhododendron henanense subsp. lingbaoense (Rhl). Rhl seedlings were treated at 40℃ (RLH), 32℃ (RLM), and 24℃ (RLC), and the changes in transcriptome and metabolome were compared. Overall, 78 differentially expressed metabolites were detected, and 8450 differentially expressed genes (DEGs) were identified. KEGG analysis revealed that the DEGs in RLH vs. RLC were mainly enriched in photosynthesis, secondary metabolic biosynthesis, and flavonoid biosynthesis. Most genes encoding glutathione-S-transferase were upregulated, whereas genes related to heat shock proteins were significantly downregulated. 31 genes related to photosynthesis were significantly upregulated (P-value < 0.001). It was speculated that these DEGs are related to the response of Rhl to high temperature stress (HTS). Overall, 9 TF families might be the key regulators of Heat stress response pathways in Rhl. Mining of DEGs revealed that the expression of some genes related to heat stress function increased highly significantly, e.g., the Rhe008987 related to Glutathione-S-transferase, Rhe016769 encoding peroxidase, and Rhe001827 encoding chalcone and stilbene synthases. Metabolome and transcriptome correlation analysis revealed that three comparison groups (RLH vs. RLC, RLH vs. RLM, and RLM vs. RLC) shared 12 metabolic pathways in which the DEMs were enriched. HTS inhibited or induced expression of genes in flavonoid biosynthesis pathway and led to decreace in kaempferol content and quercetin accumulation. HT induced expression of genes in ABC pathway, which may be one of the reasons for the significant accumulation of L-isoleucine, L-leucine, and L-proline. In this study, DEGs mining found that the expression of some genes related to heat stress function increased highly significantly. And two omics correlation analysis revealed that 12 metabolic pathways were enriched in three comparison groups. These results helped in elucidating the molecular mechanisms of response of Rhl to HTS.

Dataset Information

Characterization of the complete chloroplast genome of Rhododendron henanense subsp. lingbaoense Fang.

Publications

Characterization of the complete chloroplast genome of <i>Rhododendron henanense</i> subsp. <i>lingbaoense</i> Fang.

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