Morphological and molecular characterization of Bitylenchus hispaniensis (Nematoda: Telotylenchidae) from Iran
Ontology highlight
ABSTRACT:
SUBMITTER: Abdolkhani A
PROVIDER: S-EPMC8571903 | biostudies-literature |
REPOSITORIES: biostudies-literature
Similar Datasets
Project description:A new species of the genus Paurodontella, P. composticola n. sp., collected from Nazar Abad City, Alborz Province, Iran, is described and illustrated. The new species has a body length of 803-1053???m (females n?=?10) and 620 and 739???m (males n?=?2). The cuticle is weakly annulated with four lateral lines. Cephalic region is annulated and continuous with body contour. The stylet is 8.0 to 9.0???m long with asymmetrical knobs. Esophageal basal bulb is present with a small posterior extension projecting into the intestine. Excretory pore is situated at the level of esophageal basal bulb region. Post-uterine sac is 5 to 8???m long and uterus is without diverticulum. Tails of both sexes are similar, short and sub-cylindrical. Males have 24 to 25???m long bursa leptoderan and spicules 24 or 25?µm long. A non-branching oviduct is present to form a uterine diverticulum; the new species is closely related to five known species of the genus, namely P. asymmetrica, P. balochistanica, P. densa, P. iranica and P. niger. It most closely resembles P. iranica, but differs from it morphologically by a shorter stem-like extension projecting into lumen of intestine and male with sub-cylindrical tail vs conoid. In addition to morphological comparisons, the molecular phylogenetic analyses based on 733?bp of the partial sequence of 28S D2/D3 expansion segments of the large subunit rDNA gene (LSU) revealed this as a new species.A new species of the genus Paurodontella, P. composticola n. sp., collected from Nazar Abad City, Alborz Province, Iran, is described and illustrated. The new species has a body length of 803–1053???m (females n?=?10) and 620 and 739???m (males n?=?2). The cuticle is weakly annulated with four lateral lines. Cephalic region is annulated and continuous with body contour. The stylet is 8.0 to 9.0???m long with asymmetrical knobs. Esophageal basal bulb is present with a small posterior extension projecting into the intestine. Excretory pore is situated at the level of esophageal basal bulb region. Post-uterine sac is 5 to 8???m long and uterus is without diverticulum. Tails of both sexes are similar, short and sub-cylindrical. Males have 24 to 25???m long bursa leptoderan and spicules 24 or 25?µm long. A non-branching oviduct is present to form a uterine diverticulum; the new species is closely related to five known species of the genus, namely P. asymmetrica, P. balochistanica, P. densa, P. iranica and P. niger. It most closely resembles P. iranica, but differs from it morphologically by a shorter stem-like extension projecting into lumen of intestine and male with sub-cylindrical tail vs conoid. In addition to morphological comparisons, the molecular phylogenetic analyses based on 733?bp of the partial sequence of 28S D2/D3 expansion segments of the large subunit rDNA gene (LSU) revealed this as a new species.
| S-EPMC6929666 | biostudies-literature
Project description:Skin nodules of Onchocerca fasciata Railliet and Henry, 1910 (Spirurida, Onchocercidae) are a common finding in dromedary camels, though with a minimal clinical impact. There is little information about the morphology, molecular make-up and pathological impact of this parasite. Onchocerca fasciata nodules (1.3-2.1 cm in diameter and 509-841 mg in weight) were detected on the neck region in 31.5% of dromedary camels examined in Kerman province, southeastern Iran. Of 38 isolated nodules, only 23 (60.5%) contained viable worms. Measurement and morphological analyses were performed on isolated female worms by light microscopy. The identification of O. fasciata specimens was confirmed by sequence analysis of two mitochondrial genes (12S rDNA and cox1), which showed 0.4% divergence from available O. fasciata sequences. In addition, a phylogeny of filarial nematodes was constructed, based on these two mitochondrial genes and five nuclear genes (18S rDNA, 28S rDNA, MyoHC, rbp1, hsp70); this indicated that O. fasciata belongs to clade ONC3 of Onchocercidae, with representatives of the genera Onchocerca and Dirofilaria. Within the genus Onchocerca, O. fasciata is grouped with bovine parasitic species and the human parasitic Onchocerca volvulus, which suggests an impact of domestication on the radiation of the genus. Data provided here on the distribution and morphology of O. fasciata contribute to the molecular identification and phylogenetic position of the species.
| S-EPMC6146669 | biostudies-literature
Project description:Aphelenchoides fuchsi sp. n. is described and illustrated from bark and wood samples of a weakened Mondell pine in Kermanshah Province, western Iran. The new species has body length of 332 to 400 µm (females) and 365 to 395 µm (males). Lip region set off from body contour. The cuticle is weakly annulated, and there are four lines in the lateral field. The stylet is 8 to 10 ?m long and has small basal swellings. The excretory pore is located ca one body diam. posterior to metacorpus valve or 51 to 62 ?m from the head. The postuterine sac well developed (60-90 µm). Spicules are relatively short (15-16 ?m in dorsal limb) with apex and rostrum rounded, well developed, and the end of the dorsal limb clearly curved ventrad like a hook. The male tail has usual three pairs of caudal papillae (2+2+2) and a well-developed mucro. The female tail is conical, terminating in a complicated step-like projection, usually with many tiny nodular protuberances. The new species belongs to the Group 2 sensu Shahina, category of Aphelenchoides species. Phylogenetic analysis based on small subunit (SSU) and partial large subunit (LSU) sequences of rRNA supported the morphological results.
| S-EPMC4859616 | biostudies-literature
Project description:BackgroundBaylisascaris devosi is an intestinal nematode found in several carnivores including fisher, wolverine, Beech marten, American marten and sable in different parts of the world, but this nematode has not been reported from Pine marten. Therefore, this study aimed to identify Baylisascaris isolated from a Pine marten in Iran using morphological and molecular approaches.MethodsSpecimens of B. devosi were collected from one road-killed Pine marten in northern Iran. Morphological features were evaluated using scanning electron microscopy, energy dispersive x-ray analysis and ion sectioning. The molecular characterization was carried out using partial Cox1, LSU rDNA and ITS-rDNA genes.ResultsThe nematodes isolated from the Pine marten were confirmed to be B. devosi based on the morphological features and the sequence of ribosomal and mitochondrial loci. X-ray scans (EDAX) were completed on gallium cut structures (papillae, eggs, male spike and mouth denticles) of B. devosi using a dual-beam scanning electron microscope. The male spike and mouth denticles had a high level of hardening elements (Ca, P, S), helping to explain the chemical nature and morphology of the worm. Based on these genetic marker analyses, our sequence had the greatest similarity with Russian B. devosi isolated from sable.ConclusionsIn this study, to our knowledge, the occurrence of B. devosi infection in Pine marten is reported for the first time. Molecular analysis showed that these three genes are suitable molecular markers for identification and inferring phylogenetic relationships of Baylisascaris species. Furthermore, the high divergence of Cox1 between Baylisascaris species indicates that Cox1 could be used for their phylogenetic and taxonomic studies.
| S-EPMC7791847 | biostudies-literature
Project description:Three species of the genus Neothada, including N. cancellata, N. hades and N. major, collected from the rhizosphere of mosses in Khuzestan, southwestern Iran, are redescribed and illustrated. Neothada hades and N. major are new records from Iran. Neothada hades has 14 longitudinal incisures excluding the lateral field, body length of 586 (505-674) µm, stylet 10.5 (10.0-10.8) µm in length bearing distinct basal knobs, and an elongated-conical tail 70.4 (65-74) µm long with a finely to bluntly rounded terminus. N. major possesses 18-20 longitudinal incisures excluding the lateral field, body length of 657 (600-728) µm, stylet 10.9 (10.3-11.7) µm long with basal swellings but not distinct knobs, and an elongated-conical tail 78.2 (70-83) µm long ending to a finely to bluntly rounded terminus. Molecular phylogenetic studies of the two species (N. hades and N. major) with 664 bp of D2-D3 expansion segments of 28 S rDNA revealed that they form a clade with N. cancellata.
| S-EPMC8015382 | biostudies-literature
Project description:Pratylenchus lentis n. sp. parasitizing roots of lentil in Sicily, Italy, is described and illustrated. The new species is characterized by a relatively high lip region with three annuli, mean stylet length of 16 mum, with anteriorly flattened knobs, cylindrical body with a relatively anterior vulva, large and ovoid spermatheca full of sperm, plump tail with truncate, irregularly annulated terminus, and by the presence of males. Molecular ITS-RFLP and sequencing analyses of the new species showed clear differences from other most morphologically similar species, such as P. thornei and P. mediterraneus. Preliminary host range tests revealed that chickpea, pea, faba bean and durum wheat are good hosts of P. lentis n. sp., whereas common bean, alfalfa and barley are less robust hosts and tomato, bell pepper, eggplant, melon and sunflower are poor hosts for the nematode.
| S-EPMC2664672 | biostudies-literature
Project description:Coslenchus paramaritus n. sp. and C. cancellatus were identified based on morphological, morphometric, and molecular characteristics. The new species, C. paramaritus n. sp., is characterized by its cuticle with 18 longitudinal ridges excluding lateral lines, wide cuticular annuli (2.6-3.0 µm), a long tail (94-128 µm), the presence of males and the absence of sexual dimorphism in head shape. Molecular phylogenetic studies of the new species using D2-D3 expansion segments of 28S rRNA revealed that the new species formed a sister clade with three unknown populations of Coslenchus in Bayesian inference (BI) phylogeny, while C. cancellatus formed a sister clade with other Coslenchus species including C. oligogyrus, C. franklinae, C. costatus, C. turkeyensis and three unknown populations. C. cancellatus is recovered from Iran for the first time.
| S-EPMC6909032 | biostudies-literature
Project description:BackgroundInterest in Tatera indica rodent arises mostly because it is believed that this species is survived among four subspecies reported from Iran, two of which exist in Khuzestan Province. In addition, it might has a role as reservoir hosts of zoonotic cutaneous leishmaniasis in the transmission of Leishmania major in some of the widespread Asian foci including southwestern Iran.MethodsDiagnostic morphological and molecular markers for T. indica were sought by characterizing from individual specimens, such as some taxonomic features and mitochondrial cytochrome b gene that had previously proven useful for the taxonomy of rodents. Wild rodents were caught using live wooden and wire traps. The specimens were identified morphologically using external criteria and molecularly by sequencing of Cyt b gene and phylogenetic analyses.ResultsForty one T. indica were collected and identified morphologically in Khuzestan Province, Iran. Two morphotypes of T. indica were found and classified but sequencing and phylogenetic analyses of mitochondrial Cyt b gene did not support any subspecies between two morphotypes of T. indica. Because all 21 sequences of both morphotypes of T. indica had no variation with only one common and novel haplotype (GenBank accession No KP001566).ConclusionThis is the first time that T. indica was characterized molecularly in Iran. There is no molecular evidence for T. indica morphotypes or subspecies, and so a population genetics approach using several polymorphic genes might be employed using species-specific molecular markers. In addition, more specimens of T. indica species in large geographical locations should be tested.
| S-EPMC4813394 | biostudies-literature
Project description:A total of six soil samples were collected around rhizosphere of citrus plants during 2010 from Melkassa Agricultural Research Center experimental station, Ethiopia. From these samples two most important ecto-plant parasitic nematodes of the genus Xiphinema were found and analysed. The genus Xiphinema is a large group of the phylum nematoda which constitutes more than 260 species. They are polyphagous root- ectoparasites of many crop plants and some species of this genus cause damage by direct feeding on root tips and transmit nepoviruses. The delimitation and discrimination of two species in the genus is presented, described herein as Xiphinemaelongatum and Xiphinemapachtaicum. Morphological and morphometric data were done using light microscopy and results of both species were fit within the previously described nematode species of Xiphinemaelongatum and Xiphinemapachtaicum. 18S rDNA were analysed using Bayesian inference (BI) method to reconstruct phylogenetic relationships of the studied Xiphinema sp. (KP407872Xiphinemaelongatum and KP407873Xiphinemapachtaicum) with other Xiphinema species. The 18S rDNA sequence of Xiphinemapachtaicum was alike to previously described species from the GenBank but Xiphinemaelongatum exhibited very small levels of nucleotides differences (0.4%) which might be possible intra-specific divergence. Though this region of rDNA has less resolution on complex species, its combination with morphological and morphometric analyses, suggests these species as Xiphinemaelongatum and Xiphinemapachtaicum with the GenBank accession number of KP407872 and KP407873, respectively. Short notes, morphological measurements, illustrations, and molecular data are given to these species. These species are reported for the first time from Ethiopia and it provides new geographical information of these organisms.
| S-EPMC4395759 | biostudies-literature
Project description:Labrys filiformis n. sp., the second species of the rare genus Labrys , was recovered from natural forests of Gilan province and is described based upon morphological and molecular characters. The new species is characterized by its smooth cuticle under light microscopy, lateral field with two incisures forming a single plain band, lip region continuous with body contour, dorso-ventrally flattened and forming four poorly prominent lobes, having a dorso-ventrally narrower protuberant labial plate laterally extended to the amphidial margins, oral area (oral plate) dorso-ventrally elongated and embedded in the labial plate with six small labial sensilla surrounding the slightly prominent oral aperture, amphidial apertures as longitudinally lemniscatic slits bordered by the labial plate extensions which are overlapped at the middle length of amphids, stylet delicate, 6 to 7?µm long, elongate weakly developed fusiform median bulb with weak valve, wide excretory pore with long and heavily sclerotized duct, offset spermatheca filled with small spheroid sperm cells, 106 to 127?µm long elongate-conoid tail with filiform distal region and finely rounded tip. Molecular phylogenetic analyses were performed using a near-full length fragment of the 18S rDNA and the D2-D3 expansion segments of the 28S rDNA using Bayesian inference and maximum likelihood methods. In the inferred phylogenetic tree with 18S rDNA, the new species has a close affinity with several isolates of the type species, Labrys chinensis . The reconstructed phylogenetic tree using partial 28S rDNA, revealed the new species is nested inside the putative monophyletic group of several populations of L. chinensis .
| S-EPMC6909355 | biostudies-literature