Peripheral arterial disease: Effects of ethanolic extracts of seed kernels of mango (Mangifera indica .L) on acute hind limb ischemia-reperfusion injury in diabetic rats

ABSTRACT:

Background and aim

Hind limb ischemia is one of the peripheral arterial diseases affecting majority of the people with atherosclerosis, diabetes and chronic cigarette smokers. Hind limb ischemic-reperfusion injury is also one of the exacerbating events in these peoples, resulting in hind limb dysfunction. The aim of this study was to identify the effects of ethanolic extracts of mangifera indica (EEMI) on reversing hind limb dysfunction in diabetic rats with acute hind limb ischemia-reperfusion injury.

Experimental procedure

Unilateral femoral artery ligated diabetic rats were orally fed with EEMI (0.2 and 0.4 g/kg) for 14 days. At the end of the study, plasma levels of pro-inflammatory cytokines and relevant biochemical parameters were measured. The isolated gastrocnemius muscles were used for gene expression and histopathological studies.

Results

There was a significant reduction (p < 0.05) in the plasma levels of pro-inflammatory cytokines, nitric oxide, malondialdehyde; and the expression levels of mRNA of induced nitric oxide synthase and intercellular adhesion molecule −1; and increase in anti-inflammatory cytokine, in isolated gastrocnemius muscles of animals treated with 0.2 and 0.4 g/kg of EEMI in comparison to disease control. In addition, histopathological study of gastrocnemius muscle and hind limb function test indicated the recovery of tissue damage from ischemic reperfusion at 0.2 and 0.4 g/kg of EEMI in comparison to disease control.

Conclusion

We conclude that 14-day EEMI treatment of rats with acute hind limb ischemia/reperfusion in diabetic rats recovered from ischemic/reperfusion injury by modulating (decreasing) oxidative stress and inflammation. Graphical abstract Image 1 Highlights • Ethanolic extracts of M.indica (EEMI) was effective in diabetic rats with acute hind limb ischemia-reperfusion (AHLI-R) injury.• EEMI attenuated plasma levels of pro-inflammatory cytokines and augmented IL-10 in diabetic rats with AHLI-R injury.• EEMI augmented cell-cell adhesion molecule, ICAM-1 in gastrocnemius muscle of diabetic rats with AHLI-R injury.• EEMI reversed I/R injury and hind limb dysfunction by modulating mechanisms of oxidative stress and inflammation.

SUBMITTER: RamPravinKumar M 

PROVIDER: S-EPMC8572715 | biostudies-literature |

REPOSITORIES: biostudies-literature