High-throughput Microwell-seq 2.0 profiles massively multiplexed chemical perturbation
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ABSTRACT:
SUBMITTER: Chen H
PROVIDER: S-EPMC8575926 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:Combining in-cell RT and Microwell-seq 1.0, we established Microwell-seq 2.0 for cost-effective HTS with scRNA-seq-based phenotyping. To assess the fidelity of the Microwell-seq 2.0, we performed a species-mixing experiment with cultured human (293T) and mouse (3T3) cells. Moreover, we assessed the platform on tissue cells with more heterogeneous cell types. By harnessing the power of Microwell-seq 2.0, we analyzed massively multiplexed chemical perturbation of human embryonic stem cells (hESCs) at the single-cell resolution.
2021-11-09 | GSE175413 | GEO
Project description:High-throughput Microwell-seq 2.0 reveals massively multiplexed chemical perturbation
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