Trichostatin A enhances radiosensitivity and radiation-induced DNA damage of esophageal cancer cells.

ABSTRACT:

Background

Trichostatin A (TSA) is emerging as a potential component of anticancer therapy. In this study, we aimed to identify the radiosensitizing effects of TSA in esophageal squamous carcinoma cell lines and identify the genomic alteration of histone acetylation associated with TSA treatment.

Methods

EC109 and KYSE450 cells were pretreated with TSA (0.1 µM) for 12 hours prior to irradiation, and the cell viability, flow cytometry, and comet assays were performed to analyze cell growth, cell apoptosis, and DNA damage, respectively. Chromatin immunoprecipitation sequencing (ChIP-Seq) was performed to identify the acetylation sites of histone H3 lysine 9 (H3K9), which was altered by TSA.

Results

Our data showed that TSA could sensitize esophageal cancer cells to radi

SUBMITTER: Wang S 

PROVIDER: S-EPMC8576220 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature