Effects and molecular mechanisms of <i>Achyranthes bidentata</i> polypeptide k on proliferation of Schwann cells.

ABSTRACT:

Background

Achyranthes bidentata polypeptide k (ABPPk) is an active ingredient separated from the Achyranthes bidentata polypeptides (ABPP) in traditional Chinese medicine. In the present study, we investigated the promoting effects and molecular mechanisms of ABPPk on the proliferation of Schwann cells (SCs).

Methods

Primary SCs were cultured with ABPPk or nerve growth factor (NGF) in vitro, and cell viability, cell cycle, EdU assay, and the expressions of proliferating cell nuclear antigen (PCNA) and Ki67 were analyzed. In addition, RNA-seq was used for bioinformatics analysis at different time points. PCNA was detected at different time points in a rat sciatic nerve injury model to further determining the role of ABPPk in sciatic nerve injury repair.<

SUBMITTER: Tang L 

PROVIDER: S-EPMC8576723 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature