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A live-imaging protocol to track cell movement in the Xenopus embryo

ABSTRACT: Summary Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded. For complete details on the use and execution of this protocol, please refer to Chuyen et al. (2021). Graphical abstract Highlights • Tracking of individual cell movement in vivo during Xenopus embryonic development• Labeling and tracking of multiciliated cells (MSSs) in bilayered Xenopus epidermis• In silico stabilization corrects drifting caused by embryo morphogenesis Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells. We describe here a protocol to track immature multiciliated cells (MCCs) moving within the inner epidermal layer of a whole embryo. In addition, we present a data processing protocol to uncouple the movements of individual cells from the coplanar drifts of the tissue in which they are embedded.

SUBMITTER: Chuyen A

PROVIDER: S-EPMC8577151 | biostudies-literature |

REPOSITORIES: biostudies-literature

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