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MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A.


ABSTRACT:

Objective

The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC).

Methods

Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p in vitro was tested by a set of assays. Interaction between microRNA-139-5p and MEX3A was validated by dual-luciferase detection.

Results

MicroRNA-139-5p expression in BC cells was obviously low, while MEX3A was significantly overexpressed. MicroRNA-139-5p restrained proliferative, invasive, and migratory abilities of BC cells and increased apoptosis level of BC cells, while MEX3A exerted a promoting effect on BC cell growth. Dual-luciferase reporter detection confirmed that microRNA-139-5p bound to MEX3A 3'-UTR.

Conclusions

MicroRNA-139-5p inhibited the development of BC by targeting MEX3A. MicroRNA-139-5p/MEX3A may be a target for BC therapy.

SUBMITTER: Chu J 

PROVIDER: S-EPMC8577887 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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MicroRNA-139-5p Suppresses Cell Malignant Behaviors in Breast Cancer through Targeting MEX3A.

Chu Jian J   Li Tangya T   Li Lei L   Fan Huiwen H  

Computational and mathematical methods in medicine 20211102


<h4>Objective</h4>The study was designed to evaluate the underlying mechanism of microRNA-139-5p in breast cancer (BC).<h4>Methods</h4>Expression statuses of microRNA-139-5p and MEX3A were measured by qRT-PCR and western blotting. The anticancer effect of microRNA-139-5p <i>in vitro</i> was tested by a set of assays. Interaction between microRNA-139-5p and MEX3A was validated by dual-luciferase detection.<h4>Results</h4>MicroRNA-139-5p expression in BC cells was obviously low, while MEX3A was si  ...[more]

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