Dataset Information

Genetic evaluation of ESBL-producing Escherichia coli urinary isolates in Otago, New Zealand.

ABSTRACT:

Objectives

The incidence of infections with ESBL-producing Escherichia coli (ESBL-Ec) in New Zealand is increasing. ESBL-Ec most commonly cause urinary tract infections and are seen in both community and hospitalized patients. The reason for the increasing incidence of ESBL-Ec infections is unknown.

Methods

In this study, 65 urinary ESBL-Ec isolates from the Otago region in 2015 were fully genetically characterized to understand the mechanisms of transmission. The ESBL gene, E. coli STs, plasmid types and genetic context (e.g. insertion sequences) of ESBL genes were determined by a combination of whole genome and plasmid sequencing. The phylogenetic relationships of the isolates were compared with ESBL-Ec isolates sequenced as part of the 2016 nationwide survey.

Results

Significant diversity of E. coli strains, plasmids, and the genetic context of ESBL genes was seen. However, there was evidence of common mobile genetic elements in unrelated ESBL-Ec.

Conclusions

Multiple introductions of ESBL resistance genes or resistant bacterial strains with limited horizontal transmission of mobile genetic elements accounts for the increased incidence of ESBL-Ec in this low prevalence area. Future studies should investigate modes of transmission of ESBL-Ec in the Otago region.

SUBMITTER: Hapuarachchi IU

PROVIDER: S-EPMC8578626 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Publications

Genetic evaluation of ESBL-producing <i>Escherichia coli</i> urinary isolates in Otago, New Zealand.

Hapuarachchi Isuri U IU Hannaway Rachel F RF Roman Tabatha T Biswas Ambarish A Dyet Kristin K Morgan Xochitl X Ussher James E JE

JAC-antimicrobial resistance 20211110 4

<h4>Objectives</h4>The incidence of infections with ESBL-producing <i>Escherichia coli</i> (ESBL-Ec) in New Zealand is increasing. ESBL-Ec most commonly cause urinary tract infections and are seen in both community and hospitalized patients. The reason for the increasing incidence of ESBL-Ec infections is unknown.<h4>Methods</h4>In this study, 65 urinary ESBL-Ec isolates from the Otago region in 2015 were fully genetically characterized to understand the mechanisms of transmission. The ESBL gene ...[more]

PMID: 34778763

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