Ontology highlight
ABSTRACT:
SUBMITTER: Skladny H
PROVIDER: S-EPMC85831 | biostudies-literature | 1999 Dec
REPOSITORIES: biostudies-literature
Skladny H H Buchheidt D D Baust C C Krieg-Schneider F F Seifarth W W Leib-Mösch C C Hehlmann R R
Journal of clinical microbiology 19991201 12
The increasing incidence of aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the currently limited diagnostic tools. We developed a two-step PCR assay that specifically amplifies a region of the 18S rRNA gene that is highly conserved in Aspergillus species. A number of primers with the least homology to equivalent human or Candida gene sequences were screened for the pairs that gave the highest sensitivity and specificity. No cross-reactio ...[more]