Latently KSHV-Infected Cells Promote Further Establishment of Latency upon Superinfection with KSHV.

ABSTRACT: Kaposi's sarcoma-associated herpesvirus (KSHV) is a cancer-related virus which engages in two forms of infection: latent and lytic. Latent infection allows the virus to establish long-term persistent infection, whereas the lytic cycle is needed for the maintenance of the viral reservoir and for virus spread. By using recombinant KSHV viruses encoding mNeonGreen and mCherry fluorescent proteins, we show that various cell types that are latently-infected with KSHV can be superinfected, and that the new incoming viruses establish latent infection. Moreover, we show that latency establishment is enhanced in superinfected cells compared to primary infected ones. Further analysis revealed that cells that ectopically express the major latency protein of KSHV, LANA-1, prior to and during infection

SUBMITTER: Gam Ze Letova C 

PROVIDER: S-EPMC8584431 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature