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A manganese (II)-based coordinative dendrimer with robust efficiency in intracellular peptide delivery

ABSTRACT: Peptides have gained increasing interests as drug candidates in modern pharmaceutical industry, however, the development of peptide drugs acting on intracellular targets is limited due to their membrane impermeability. Here, we reported the use of metal-terpyridine based coordinative dendrimer for cytosolic peptide delivery. Among the investigated transition metal ions, Mn2+-coordinated polymer showed the highest delivery efficiency due to balanced peptide binding and release. It showed robust efficiency in the delivery of peptides with different charge property and hydrophobicity into various primary cells. The efficiency of Mn2+-terpyridine based polymer is superior to cell penetrating peptides such as oligoarginines. The material also delivered an autophagy-inducing peptide derived from Beclin-1 into cells and efficiently induced autophagy in the cells. This study provides a promising alternative to cell penetrating peptides for cytosolic peptide delivery. Graphical abstract Image 1 Highlights • A Mn2+/terpyridine based polymer is rationally designed for cytosolic peptide delivery.• The polymer shows robust efficiency in the delivery of 22 peptides with different properties into various primary cells.• The polymer delivers an autophagy-inducing peptide derived from Beclin-1 into cells and efficiently induces autophagy.• This study provides a promising alternative to cell penetrating peptides for cytosolic peptide delivery.

SUBMITTER: Ren L

PROVIDER: S-EPMC8586439 | biostudies-literature |

REPOSITORIES: biostudies-literature

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