CDK1、CCNB1和NDC80与乙型肝炎相关肝细胞癌的预后和进展相关:基于生物信息学方法 CDK1, CCNB1 and NDC80 are associated with prognosis and progression of hepatitis B virus-associated hepatocellular carcinoma: a bioinformatic analysis
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ABSTRACT: 目的
探讨HBV转化为HCC相关的关键基因，并探索相关的分子机制。 方法
分析基因表达综合（GEO）数据库中GSE55092、GSE84044和GSE121248的mRNA微阵列数据，其中包括119个HBV相关的HCC组织和252个HBV相关的非肿瘤组织。“sva” R包用于去除批间差。进行整合分析，获取HBV相关肝癌和HBV组织中的差异表达基因（DEGs），通过基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析对差异表达基因进行功能注释。使用相互作用基因搜索工具（STRING）构建蛋白质-蛋白质相互作用网络，挖掘最重要的模块和关键基因。cBioportal用于分析hub基因的相关性。利用Kaplan-Meier和Oncomine数据库对TCGA数据库中肝癌基因表达数据进行验证，探讨hub基因与肝癌发生、发展和预后的关系。通过逆转录定量PCR（RT-qPCR）实验验证17对临床肝细胞癌样本与邻近非肿瘤组织中hub基因的表达。 结果
共获得121个DEGs（P＜0.01），鉴定出3个遗传标记，细胞周期素依赖性激酶1（CDK1）、细胞周期蛋白B1（CCNB1）和核分裂周期蛋白80（NDC80），与细胞周期、嘧啶代谢和DNA复制有关，这3个基因高度相关（P＜0.05）。UALCAN数据库证实这些基因在肝癌组织中高表达并获得相关预后信息。Kaplan-Meier表明，它们与HCC患者的低存活率相关。CDK1、CCNB1和NDC80与肝癌分级相关（P＜0.05），RT-qPCR实验证实CDK1、CCNB1和NDC80的mRNA在肝细胞癌中的表达明显高于癌旁组织。 结论
CDK1、CCNB1和NDC80基因可作为HBV相关肝细胞癌的预后标志物，在肝癌的基础研究和临床治疗方面有一定的应用价值。
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PROVIDER: S-EPMC8586853 | biostudies-literature |
REPOSITORIES: biostudies-literature
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