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Production and Characterization of Polyethylene Terephthalate Nanoparticles

ABSTRACT: Microplastic (MP) pollution represents one of the biggest environmental problems that is further exacerbated by the continuous degradation in the marine environment of MPs to nanoplastics (NPs). The most diffuse plastics in oceans are commodity polymers, mainly thermoplastics widely used for packaging, such as polyethylene terephthalate (PET). However, the huge interest in the chemical vector role of micro/nanoplastics, their fate and negative effects on the environment and human health is still under discussion and the research is still sparse due also to the difficulties of sampling MPs and NPs from the environment or producing NPs in laboratory. Moreover, the research on MPs and NPs pollution relies on the availability of engineered nanoparticles similar to those present in the marine environment for toxicological, transport and adsorption studies in biological tissues as well as for wastewater remediation studies. This work aims to develop an easy, fast and scalable procedure for the production of representative model nanoplastics from PET pellets. The proposed method, based on a simple and economic milling process, has been optimized considering the peculiarities of the polymer. The results demonstrated the reliability of the method for preparing particle suspensions for aquatic microplastic research, with evident advantages compared to the present literature procedures, such as low cost, the absence of liquid nitrogen, the short production time, the high yield of the process, stability, reproducibility and polydisperse size distribution of the produced water dispersed nanometric PET.

SUBMITTER: Lionetto F

PROVIDER: S-EPMC8587476 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Biodegradation of terephthalate (TPA) is a highly desired catabolic process for the bacterial utilization of this polyethylene terephthalate (PET) depolymerization product, but to date, the structure of terephthalate dioxygenase (TPDO), a Rieske oxygenase (RO) that catalyzes the dihydroxylation of TPA to a cis-diol, is unavailable. In this study, we characterized the steady-state kinetics and first crystal structure of TPDO from Comamonas testosteroni KF1 (TPDOKF1). TPDOKF1 exhibited substrate specificity for TPA (kcat/Km = 57 ± 9 mM-1 s-1). The TPDOKF1 structure harbors characteristic RO features as well as a unique catalytic domain that rationalizes the enzyme's function. The docking and mutagenesis studies reveal that its substrate specificity for TPA is mediated by the Arg309 and Arg390 residues, positioned on opposite faces of the active site. Additionally, residue Gln300 is also proven to be crucial for the activity, as its mutation to alanine decreases the activity (kcat) by 80%. This study delineates the structural features that dictate the substrate recognition and specificity of TPDO. IMPORTANCE Global plastic pollution has become the most pressing environmental issue. Recent studies on enzymes depolymerizing polyethylene terephthalate plastic into terephthalate (TPA) show some potential for tackling this. Microbial utilization of this released product, TPA, is an emerging and promising strategy for waste-to-value creation. Research in the last decade has identified terephthalate dioxygenase (TPDO) as being responsible for initiating the enzymatic degradation of TPA in a few Gram-negative and Gram-positive bacteria. Here, we determined the crystal structure of TPDO from Comamonas testosteroni KF1 and revealed that it possesses a unique catalytic domain featuring two basic residues in the active site to recognize TPA. Biochemical and mutagenesis studies demonstrated the crucial residues responsible for the substrate specificity of this enzyme.

Project description:In this research, various emulsions of copper-based nanoparticles were synthesized through the chemical reduction method followed by utilizing the pad–dry–cure technique as an industrial approach to manufacturing bactericidal polyethylene terephthalate (PET) substrates. Copper sulfate/copper acetate, sodium hypophosphite (SHP)/ascorbic acid, and cetyltrimethylammonium bromide were employed as salts, reducing agents, and stabilizers, respectively. Also, a spin finish oil was used for forming an emulsion. The effects of type and amount of copper salt and reductant as well as the use of resin and stabilizer were investigated concerning antibacterial activities, weight, and color changes of coated samples to find optimum formulation. Field-emission scanning electron microscope (FESEM) images, mapping/energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD) pattern, Raman spectroscopy, and UV–visible spectrophotometer was proved successful in synthesis and loading of copper-based emulsions on the PET substrates. The results revealed that change of copper salt, substituting SHP with ascorbic acid, the addition of resin, and the use of surfactant yielded negligible effect, enhancing impact, reducing the influence, and improving efficacy on bactericidal characteristics of the treated samples, respectively. Based on findings, the samples coated by emulsion containing only copper sulfate/SHP and emulsion including only copper acetate were considered optimum samples indicating 100% bactericidal properties against both S. aureus and E. coli pathogenic bacteria. Despite showing bactericidal activities, it was further found that the treated samples exhibited cell toxicity toward human skin cells implying their applications in indirect contact usages. Coated samples further indicated a good washing fastness even after 20 washing cycles. This route can be considered as a facile industrially applicable method for imparting bactericidal properties to polymeric substrates. Furthermore, such emulsions can potentially be consumed as an antibacterial spin finish oil in melt-spinning to develop antibacterial textiles. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s12221-023-00047-5.

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Production and Characterization of Polyethylene Terephthalate Nanoparticles

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