Purification and Characterization of an Extracellular Alkaline Solvent-stable Metalloprotease Secreted from Newly Isolated Bacillus sp. DEM05: Optimization of Protease Production
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ABSTRACT: Background:
Proteases play an important role in food, leather, detergent, and medical technologies. Objectives:
In the current study, an alkaliphilic solvent-stable thermotolerant metalloprotease was isolated from Bacillus sp. DEM05. Material and Methods:
For culture optimization, carbon, and nitrogen sources as well as incubation temperature, pH, and time were examined. Results:
Herein the highest outcome for bacterial growth and protease production was obtained after 72 h incubation (pH 7) at 37 °C. DEM05 protease
SUBMITTER: Mohamadi S
PROVIDER: S-EPMC8590717 | biostudies-literature |
REPOSITORIES: biostudies-literature
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