The mammalian rod synaptic ribbon is essential for Ca_v channel facilitation and ultrafast synaptic vesicle fusion.

ABSTRACT: Rod photoreceptors (PRs) use ribbon synapses to transmit visual information. To signal 'no light detected' they release glutamate continually to activate post-synaptic receptors. When light is detected glutamate release pauses. How a rod's individual ribbon enables this process was studied here by recording evoked changes in whole-cell membrane capacitance from wild type and ribbonless (Ribeye-ko) mice. Wild type rods filled with high (10 mM) or low (0.5 mM) concentrations of the Ca²⁺-buffer EGTA created a readily releasable pool (RRP) of 87 synaptic vesicles (SVs) that emptied as a single kinetic phase with a τ < 0.4 msec. The lower concentration of EGTA accelerated Ca_v channel opening and facilitated release kinetics. In contrast, ribbonless rods created a much smaller RRP of 22 SVs, and they lacked Ca_v channel facilitation; however, Ca²⁺ channel-release coupling remained tight. These release deficits caused a sharp attenuation of rod-driven light responses. We conclude that the synaptic ribbon facilitates Ca²⁺-influx and establishes a large RRP of SVs.

SUBMITTER: Grabner CP 

PROVIDER: S-EPMC8594941 | biostudies-literature |

REPOSITORIES: biostudies-literature