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Singlet-Oxygen-Induced Phospholipase A₂ Inhibition: A Major Role for Interfacial Tryptophan Dioxidation.

ABSTRACT: Several studies have revealed that various diseases such as cancer have been associated with elevated phospholipase A₂ (PLA₂ ) activity. Therefore, the regulation of PLA₂ catalytic activity is undoubtedly vital. In this study, effective inactivation of PLA₂ due to reactive species produced from cold physical plasma as a source to model oxidative stress is reported. We found singlet oxygen to be the most relevant active agent in PLA₂ inhibition. A more detailed analysis of the plasma-treated PLA₂ identified tryptophan 128 as a hot spot, rich in double oxidation. The significant dioxidation of this interfacial tryptophan resulted in an N-formylkynurenine product via the oxidative opening of the tryptophan indole ring. Molecular dynamics simulation indicated that the efficient interactions between the tryptophan residue and phospholipids are eliminated following tryptophan dioxidation. As interfacial tryptophan residues are predominantly involved in the attaching of membrane enzymes to the bilayers, tryptophan dioxidation and indole ring opening leads to the loss of essential interactions for enzyme binding and, consequently, enzyme inactivation.

SUBMITTER: Nasri Z

PROVIDER: S-EPMC8596696 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Singlet-Oxygen-Induced Phospholipase A2 Inhibition: A Major Role for Interfacial Tryptophan Dioxidation.

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Singlet-Oxygen-Induced Phospholipase A₂ Inhibition: A Major Role for Interfacial Tryptophan Dioxidation.