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Inside a Shell-Organometallic Catalysis Inside Encapsulin Nanoreactors.

ABSTRACT: Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins are self-assembling protein-based nanocompartments from the prokaryotic repertoire that present a highly attractive platform for intracellular compartmentalization of chemical reactions by design. Using single-molecule Förster resonance energy transfer and 3D-MINFLUX analysis, we analyze fluorescently labeled encapsulins on a single-molecule basis. Furthermore, by equipping these capsules with a synthetic ruthenium catalyst via covalent attachment to a non-native host protein, we are able to perform in vitro catalysis and go on to show that engineered encapsulins can be used as hosts for transition metal catalysis inside living cells in confined space.

SUBMITTER: Lohner P

PROVIDER: S-EPMC8596989 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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Inside a Shell-Organometallic Catalysis Inside Encapsulin Nanoreactors.

Lohner Philipp P Zmyslia Mariia M Thurn Johann J Pape Jasmin K JK Gerasimaitė Rūta R Keller-Findeisen Jan J Groeer Saskia S Deuringer Benedikt B Süss Regine R Walther Andreas A Hell Stefan W SW Lukinavičius Gražvydas G Hugel Thorsten T Jessen-Trefzer Claudia C

Angewandte Chemie (International ed. in English) 20211001 44

Compartmentalization of chemical reactions inside cells are a fundamental requirement for life. Encapsulins are self-assembling protein-based nanocompartments from the prokaryotic repertoire that present a highly attractive platform for intracellular compartmentalization of chemical reactions by design. Using single-molecule Förster resonance energy transfer and 3D-MINFLUX analysis, we analyze fluorescently labeled encapsulins on a single-molecule basis. Furthermore, by equipping these capsules ...[more]

PMID: 34418246

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