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Optogenetic axon guidance in embryonic zebrafish.


ABSTRACT: Axons form the long-range connections of biological neuronal networks, which are built through the developmental process of axon guidance. Here, we describe a protocol to precisely and non-invasively control axonal growth trajectories in live zebrafish embryos using focal light activation of a photoactivatable Rac1. We outline techniques for photostimulation, time-lapse imaging, and immunohistochemistry. These approaches enable engineering of long-range axonal circuitry or repair of defective circuits in living zebrafish, despite a milieu of competing endogenous signals and repulsive barriers. For complete details on the use and execution of this protocol, please refer to Harris et al. (2020).

SUBMITTER: Harris JM 

PROVIDER: S-EPMC8605397 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Optogenetic axon guidance in embryonic zebrafish.

Harris James M JM   Yu-Der Wang Andy A   Arlotta Paola P  

STAR protocols 20211115 4


Axons form the long-range connections of biological neuronal networks, which are built through the developmental process of axon guidance. Here, we describe a protocol to precisely and non-invasively control axonal growth trajectories in live zebrafish embryos using focal light activation of a photoactivatable Rac1. We outline techniques for photostimulation, time-lapse imaging, and immunohistochemistry. These approaches enable engineering of long-range axonal circuitry or repair of defective ci  ...[more]

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