Ontology highlight
ABSTRACT:
SUBMITTER: Harris JM
PROVIDER: S-EPMC8605397 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Harris James M JM Yu-Der Wang Andy A Arlotta Paola P
STAR protocols 20211115 4
Axons form the long-range connections of biological neuronal networks, which are built through the developmental process of axon guidance. Here, we describe a protocol to precisely and non-invasively control axonal growth trajectories in live zebrafish embryos using focal light activation of a photoactivatable Rac1. We outline techniques for photostimulation, time-lapse imaging, and immunohistochemistry. These approaches enable engineering of long-range axonal circuitry or repair of defective ci ...[more]