Ontology highlight
ABSTRACT:
SUBMITTER: Smith EM
PROVIDER: S-EPMC8608280 | biostudies-literature | 2019 Jun
REPOSITORIES: biostudies-literature
Smith Elizabeth M EM Gautier Arnaud A Puchner Elias M EM
ACS chemical biology 20190523 6
We develop and employ the Fluorescence-Activating and absorption-Shifting Tag (FAST) system for super-resolution (SR) imaging and single-molecule tracking based on single-molecule localizations. The fast off rate of fluorogen binding, combined with its spatially well-separated labeling of the densely expressed FAST fusion proteins, allowed single-molecule measurements to be performed in both living and fixed cells. The well-separated fluorescence localization density was achieved by either rever ...[more]