Accelerated discovery of novel glycoside hydrolases using targeted functional profiling and selective pressure on the rumen microbiome.

ABSTRACT:

Background

Carbohydrate-active enzymes (CAZymes) form the most widespread and structurally diverse set of enzymes involved in the breakdown, biosynthesis, or modification of lignocellulose that can be found in living organisms. However, the structural diversity of CAZymes has rendered the targeted discovery of novel enzymes extremely challenging, as these proteins catalyze many different chemical reactions and are sourced by a vast array of microbes. Consequently, many uncharacterized members of CAZyme families of interest have been overlooked by current methodologies (e.g., metagenomic screening) used to discover lignocellulolytic enzymes.

Results

In the present study, we combined phenotype-based selective pressure on the rumen microbiota with targeted functional profiling

SUBMITTER: Neves ALA 

PROVIDER: S-EPMC8609826 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature