CRISPR-Cas12a-Based Detection of SARS-CoV-2 Harboring the E484K Mutation.
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ABSTRACT:
SUBMITTER: Marques MC
PROVIDER: S-EPMC8610009 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
CRISPR-Cas12a-Based Detection of SARS-CoV-2 Harboring the E484K Mutation.
Marqués María-Carmen MC Ruiz Raúl R Montagud-Martínez Roser R Márquez-Costa Rosa R Albert Sandra S Domingo-Calap Pilar P Rodrigo Guillermo G
ACS synthetic biology 20211116 12
The novel respiratory virus SARS-CoV-2 is rapidly evolving across the world with the potential of increasing its transmission and the induced disease. Here, we applied the CRISPR-Cas12a system to detect, without the need of sequencing, SARS-CoV-2 genomes harboring the E484K mutation, first identified in the Beta variant and catalogued as an escape mutation. The E484K mutation creates a canonical protospacer adjacent motif for Cas12a recognition in the resulting DNA amplicon, which was exploited ...[more]
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