The CRISPR-associated Cas4 protein from Leptospira interrogans demonstrate versatile nuclease activity.

ABSTRACT: The Cas4 protein is one of the core CRISPR-associated (Cas) proteins implicated in the adaptation module in many variants of the CRISPR-Cas system in prokaryotes against the invading genetic elements. Cas4 is recognized as a DNA exonuclease that contains a RecB nuclease domain and a Fe-S cluster-binding module. In Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130, the cas4 gene is functionally transcribed as an active component of the CRISPR-Cas I-B system. Investigation of nuclease activity of Cas4 (LinCas4) of the L. interrogans illustrated divalent-metal cofactor (Mn²⁺ or Mg²⁺) dependent endonuclease activity on the DNA substrate. In agreement, mutation of the selective metal interacting residues (Asp⁷⁴ and Glu^87<

SUBMITTER: Dixit B 

PROVIDER: S-EPMC8610317 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature