Flow characterization and patch clamp dose responses using jet microfluidics in a tubeless microfluidic device.

ABSTRACT:

Background

Surface tension passive pumping is a way to actuate flow without the need for pumps, tubing or valves by using the pressure inside small drop to move liquid via a microfluidic channel. These types of tubeless devices have typically been used in cell biology. Herein we present the use of tubeless devices as a fluid exchange platform for patch clamp electrophysiology.

New method

Inertia from high-speed droplets and jets is used to create flow and perform on-the-fly mixing of solutions. These are then flowed over GABA transfected HEK cells under patch in order to perform a dose response analysis.

Results

TIRF imaging and electrical recordings are used to study the fluid exchange properties of the microfluidic device, resulting in 0-90% fluid exchange times of

SUBMITTER: Resto PJ 

PROVIDER: S-EPMC8611316 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature