Unknown

Dataset Information

0

Complete genome sequence and construction of an infectious full-length cDNA clone of a cucumber vein yellowing virus (CVYV) isolate from Portugal.


ABSTRACT: Cucumber vein yellowing virus (CVYV) is a member of the genus Ipomovirus in the family Potyviridae. In the National Center for Biotechnology Information (NCBI) database, three complete genome sequences of CVYV isolates from Spain (NC_006941), Israel (KT276369), and Jordan (JF460793) are available. In this study, we report the complete sequence of an isolate of CVYV from Portugal (DSMZ PV-0776) along with the construction of an infectious full-length cDNA clone via Gibson assembly. The sequence of CVYV Portugal shows the closest relationship to a CVYV isolate from Spain (genome, 99.7% identity; polyprotein, 99.7% identity). The CVYV full-length cDNA clone was introduced by electroporation into Rhizobium radiobacter and infiltrated into the cotyledons of Cucumis sativus plantlets, resulting in symptoms resembling those of the wild-type virus. Transmission of the infectious CVYV full-length clone by the whitefly Bemisia tabaci was confirmed. This first report confirming the infectivity of a CVYV cDNA clone provides the opportunity to study gene functions in a consistent genomic background.

SUBMITTER: Cordes K 

PROVIDER: S-EPMC8616879 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC4174829 | biostudies-literature
| S-EPMC6638831 | biostudies-literature
| S-EPMC9707635 | biostudies-literature
| S-EPMC10721977 | biostudies-literature
| S-EPMC4690859 | biostudies-literature
| S-EPMC8827028 | biostudies-literature
| S-EPMC3062622 | biostudies-literature
| S-EPMC10920733 | biostudies-literature
| S-EPMC4174851 | biostudies-literature
| S-EPMC7601338 | biostudies-literature