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Identification of Renoprotective Phytosterols from Mulberry (Morus alba) Fruit against Cisplatin-Induced Cytotoxicity in LLC-PK1 Kidney Cells.


ABSTRACT: The aim of this study was to explore the protective effects of bioactive compounds from the fruit of the mulberry tree (Morus alba L.) against cisplatin-induced apoptosis in LLC-PK1 pig kidney epithelial cells. Morus alba fruit is a well-known edible fruit commonly used in traditional folk medicine. Chemical investigation of M. alba fruit resulted in the isolation and identification of six phytosterols (1-6). Their structures were determined as 7-ketositosterol (1), stigmast-4-en-3β-ol-6-one (2), (3β,6α)-stigmast-4-ene-3,6-diol (3), stigmast-4-ene-3β,6β-diol (4), 7β-hydroxysitosterol 3-O-β-d-glucoside (5), and 7α-hydroxysitosterol 3-O-β-d-glucoside (6) by analyzing their physical and spectroscopic data as well as liquid chromatography/mass spectrometry data. All compounds displayed protective effects against cisplatin-induced LLC-PK1 cell damage, improving cisplatin-induced cytotoxicity to more than 80% of the control value. Compound 1 displayed the best effect at a relatively low concentration by inhibiting the percentage of apoptotic cells following cisplatin treatment. Its molecular mechanisms were identified using Western blot assays. Treatment of LLC-PK1 cells with compound 1 decreased the upregulated phosphorylation of p38 and c-Jun N-terminal kinase (JNK) following cisplatin treatment. In addition, compound 1 significantly suppressed cleaved caspase-3 in cisplatin-induced LLC-PK1 cells. Taken together, these findings indicated that cisplatin-induced apoptosis was significantly inhibited by compound 1 in LLC-PK1 cells, thereby supporting the potential of 7-ketositosterol (1) as an adjuvant candidate for treating cisplatin-induced nephrotoxicity.

SUBMITTER: Lee D 

PROVIDER: S-EPMC8623081 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Identification of Renoprotective Phytosterols from Mulberry (<i>Morus alba</i>) Fruit against Cisplatin-Induced Cytotoxicity in LLC-PK1 Kidney Cells.

Lee Dahae D   Lee Seoung Rak SR   Park Bang Ju BJ   Song Ji Hoon JH   Kim Jung Kyu JK   Ko Yuri Y   Kang Ki Sung KS   Kim Ki Hyun KH  

Plants (Basel, Switzerland) 20211117 11


The aim of this study was to explore the protective effects of bioactive compounds from the fruit of the mulberry tree (<i>Morus alba</i> L.) against cisplatin-induced apoptosis in LLC-PK1 pig kidney epithelial cells. <i>Morus alba</i> fruit is a well-known edible fruit commonly used in traditional folk medicine. Chemical investigation of <i>M. alba</i> fruit resulted in the isolation and identification of six phytosterols (<b>1</b>-<b>6</b>). Their structures were determined as 7-ketositosterol  ...[more]

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