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Insights into Regulation of C₂ and C₄ Photosynthesis in Amaranthaceae/Chenopodiaceae Using RNA-Seq.

ABSTRACT: Amaranthaceae (incl. Chenopodiaceae) shows an immense diversity of C₄ syndromes. More than 15 independent origins of C₄ photosynthesis, and the largest number of C₄ species in eudicots signify the importance of this angiosperm lineage in C₄ evolution. Here, we conduct RNA-Seq followed by comparative transcriptome analysis of three species from Camphorosmeae representing related clades with different photosynthetic types: Threlkeldia diffusa (C₃), Sedobassia sedoides (C₂), and Bassia prostrata (C₄). Results show that B. prostrata belongs to the NADP-ME type and core genes encoding for C₄ cycle are significantly upregulated when compared with Sed. sedoides and T. diffusa. Sedobassia sedoides and B. prostrata share a number of upregulated C₄-related genes; however, two C₄ transporters (DIT and TPT) are found significantly upregulated only in Sed. sedoides. Combined analysis of transcription factors (TFs) of the closely related lineages (Camphorosmeae and Salsoleae) revealed that no C₃-specific TFs are higher in C₂ species compared with C₄ species; instead, the C₂ species show their own set of upregulated TFs. Taken together, our study indicates that the hypothesis of the C₂ photosynthesis as a proxy towards C₄ photosynthesis is questionable in Sed. sedoides and more in favour of an independent evolutionary stable state.

SUBMITTER: Siadjeu C

PROVIDER: S-EPMC8624041 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:C4 photosynthesis is a carbon-concentrating mechanism that evolved independently more than 60 times in a wide range of angiosperm lineages. Among other alterations, the evolution of C4 from ancestral C3 photosynthesis requires changes in the expression of a vast number of genes. Differential gene expression analyses between closely related C3 and C4 species have significantly increased our understanding of C4 functioning and evolution. In Chenopodiaceae, a family that is rich in C4 origins and photosynthetic types, the anatomy, physiology and phylogeny of C4, C2, and C3 species of Salsoleae has been studied in great detail, which facilitated the choice of six samples of five representative species with different photosynthetic types for transcriptome comparisons. mRNA from assimilating organs of each species was sequenced in triplicates, and sequence reads were de novo assembled. These novel genetic resources were then analyzed to provide a better understanding of differential gene expression between C3, C2 and C4 species. All three analyzed C4 species belong to the NADP-ME type as most genes encoding core enzymes of this C4 cycle are highly expressed. The abundance of photorespiratory transcripts is decreased compared to the C3 and C2 species. Like in other C4 lineages of Caryophyllales, our results suggest that PEPC1 is the C4-specific isoform in Salsoleae. Two recently identified transporters from the PHT4 protein family may not only be related to the C4 syndrome, but also active in C2 photosynthesis in Salsoleae. In the two populations of the C2 species S. divaricata transcript abundance of several C4 genes are slightly increased, however, a C4 cycle is not detectable in the carbon isotope values. Most of the core enzymes of photorespiration are highly increased in the C2 species compared to both C3 and C4 species, confirming a successful establishment of the C2 photosynthetic pathway. Furthermore, a function of PEP-CK in C2 photosynthesis appears likely, since PEP-CK gene expression is not only increased in S. divaricata but also in C2 species of other groups.

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Insights into Regulation of C₂ and C₄ Photosynthesis in Amaranthaceae/Chenopodiaceae Using RNA-Seq.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Insights into Regulation of C2 and C4 Photosynthesis in Amaranthaceae/Chenopodiaceae Using RNA-Seq.

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OmicsDI is part of the ELIXIR infrastructure

Tweets

Insights into Regulation of C₂ and C₄ Photosynthesis in Amaranthaceae/Chenopodiaceae Using RNA-Seq.