Distinguishing between Isobaric Ions Using Microdroplet Hydrogen-Deuterium Exchange Mass Spectrometry.
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ABSTRACT:
SUBMITTER: Song X
PROVIDER: S-EPMC8625015 | biostudies-literature | 2021 Oct
REPOSITORIES: biostudies-literature
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Distinguishing between Isobaric Ions Using Microdroplet Hydrogen-Deuterium Exchange Mass Spectrometry.
Song Xiaowei X Li Jia J Mofidfar Mohammad M Zare Richard N RN
Metabolites 20211023 11
Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass spectrometry (MS) alone, but this limitation can be overcome by using hydrogen-deuterium exchange (HDX) in microdroplets. Because isobaric ions may contain a varied number of exchangeable sites and different types of functional groups, each one produces a unique MS spectral pattern after droplet spray HDX without the need for MS/MS experiments or introduction of ion mobility measurements. As an example of ...[more]
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