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Fungi Associated with Postharvest Diseases of Sweet Potato Storage Roots and In Vitro Antagonistic Assay of Trichoderma harzianum against the Diseases.

ABSTRACT: Sweet potato is the 11th most important food crop in the world and an excellent source of nutrition. Postharvest diseases were monitored in sweet potato storage roots collected from the local markets in Korea during 2021. Several diseases including Fusarium surface and root rot, charcoal rot, dry rot, and soft rot were observed in the postharvest sweet potatoes. A total of 68 fungal isolates were obtained from the diseased samples, and the isolates were grouped into 8 different fungal colony types. Based on multilocus phylogeny and morphological analysis of 17 representative isolates, the isolates were identified as Fusarium oxysporum, F. ipomoeae, F. solani, Penicillium citrinum, P. rotoruae, Aspergillus wentii, Mucor variicolumellatus (Mu. circinelloides species complex), and Macrophomina phaseolina. F. oxysporum was the predominant pathogen as this is the most common pathogen of sweet potato storage roots causing the surface rot disease, and M. phaseolina caused the most severe disease among the pathogens. Dual culture antagonistic assays were evaluated using Trichoderma harzianum strains CMML20-26 and CMML20-27. The results revealed that the two strains showed strong antifungal activity in different ranges against all tested pathogens. This study provides an understanding of diverse postharvest diseases in sweet potatoes and suggests potential biocontrol agents to manage the diseases. In addition, this is the first report of sweet potato storage root rot diseases caused by A. wentii, and P. rotoruae worldwide.

SUBMITTER: Paul NC

PROVIDER: S-EPMC8625119 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:AimsIron (Fe) deficiency in soil is a continuing problem for soybean (Glycine max L.) production, partly as a result of continuing climate change. This study elucidates how Trichoderma harzianum strain T22 (TH) mitigates growth retardation associated with Fe-deficiency in a highly sensitive soybean cultivar.Methods and resultsSoil TH supplementation led to mycelial colonization and the presence of UAOX1 gene in roots that caused substantial improvement in chlorophyll score, photosynthetic efficiency and morphological parameters, indicating a positive influence on soybean health. Although rhizosphere acidification was found to be a common feature of Fe-deficient soybean, the upregulation of Fe-reductase activity (GmFRO2) and total phenol secretion were two of the mechanisms that substantially increased the Fe availability by TH. Heat-killed TH applied to soil caused no improvement in photosynthetic attributes and Fe-reductase activity, confirming the active role of TH in mitigating Fe-deficiency. Consistent increases in tissue Fe content and increased Fe-transporter (GmIRT1, GmNRAMP2a, GmNRAMP2b and GmNRAMP7) mRNA levels in roots following TH supplementation were observed only under Fe-deprivation. Root cell death, electrolyte leakage, superoxide (O2 •- ) and hydrogen peroxide (H2 O2 ) substantially declined due to TH in Fe-deprived plants. Further, the elevation of citrate and malate concentration along with the expression of citrate synthase (GmCs) and malate synthase (GmMs) caused by TH suggest improved chelation of Fe in Fe-deficient plants. Results also suggest that TH has a role in triggering antioxidant defence by increasing the activity of glutathione reductase (GR) along with elevated S-metabolites (glutathione and methionine) to stabilize redox status under Fe-deficiency.ConclusionsTH increases the availability and mobilization of Fe by inducing Fe-uptake pathways, which appears to help provide resistance to oxidative stress associated with Fe-shortage in soybean.Significance and impact of the studyThese findings indicate that while Fe deficiency does not affect the rate or degree of TH hyphal association in soybean roots, the beneficial effects of TH alone may be Fe deficiency-dependent.

Project description:Poultry manure (PM) has demonstrated its potential to enhance crop nutritional quality. Nevertheless, there remains a dearth of knowledge regarding its synergistic effects when combined with wood biochar (B) on the nutrient concentrations in sweet potato leaves (Ipomoea batatas L.) and the mineral content stored in sweet potato storage roots. Hence, a two-year field trial was undertaken during the 2019 and 2020 cropping seasons in southwestern Nigeria, spanning two locations (Owo-site A and Obasooto-site B), to jointly apply poultry manure and wood biochar as soil amendments aimed at enhancing the nutritional quality of sweet potato crop. Each year, the experiment involved different combinations of poultry manure at rates of 0, 5.0, and 10.0 t ha-1 and biochar at rates of 0, 10.0, 20.0, and 30.0 t ha-1, organized in a 3 × 4 factorial layout. The results of the present study demonstrated that the individual application of poultry manure (PM), biochar (B), or their combination had a significant positive impact on the nutrient composition of sweet potato leaves and minerals stored in the sweet potato storage roots, with notable synergistic effects between poultry manure and biochar (PM × B) in enhancing these parameters. This highlights the potential of biochar to enhance the efficiency of poultry manure utilization and improve nutrient utilization from poultry manure. The highest application rate of poultry manure at 10.0 t ha-1 and biochar at 30.0 t ha-1 (PM10 + B30), resulted in the highest leaf nutrient concentrations and mineral composition compared to other treatments at both sites. Averaged over two years, the highest application rate of poultry manure at 10.0 t ha-1 and biochar at 30.0 t ha-1 (PM10 + B30) significantly increased sweet potato leaf nutrient concentrations: nitrogen by 88.2%, phosphorus by 416.7%, potassium by 123.8%, calcium by 927.3%, and magnesium by 333.3%, compared to those in the control (PM0 + B0). The same treatment increased the concentration of sweet potato root storage minerals: phosphorus by 152.5%, potassium by 77.4%, calcium by 205.5%, magnesium by 294.6%, iron by 268.4%, zinc by 228.6%, and sodium by 433.3%, compared to the control. The highest application rate of poultry manure at 10.0 t ha-1 and biochar at 30.0 t ha-1 yielded the highest economic profitability in terms of gross margin (44,034 US$ ha-1), net return (30,038 US$ ha-1) and return rate or value-to-cost ratio (VCR) (263). The results suggested that the application of poultry manure at 10 t ha-1 and biochar at 30 t ha-1 is economically profitable in the study areas and under similar agroecological zones and soil conditions.

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Fungi Associated with Postharvest Diseases of Sweet Potato Storage Roots and In Vitro Antagonistic Assay of Trichoderma harzianum against the Diseases.

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