A 41,500 year-old decorated ivory pendant from Stajnia Cave (Poland).
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ABSTRACT:
SUBMITTER: Talamo S
PROVIDER: S-EPMC8626500 | biostudies-literature |
REPOSITORIES: biostudies-literature
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2024-04-16 | PXD051511 |
Project description:microRNAs(miRNAs) play critical regulatory roles mainly through cleaving targeted mRNAs or repressing gene translation during plant developments. Grapevine is amongst the most economically important fruit crops with whole genome available, and the study on grapevine miRNAs (Vv-miRNAs) have also been emphasized. However, the regulation mode of Vv-miRNAs on their target mRNAs during grapevine development has not been studied well, especially on a transcriptome-wide level. Here, six small RNA (sRNA) and mRNA libraries from various grapevine tissues were constructed for Illumina and Degradome sequencing. Subsequently, the spatiotemporal variation in the Vv-miRNAs’ regulation on their target genes was systematically analyzed. Totally, 242 known and 132 novel Vv-miRNAs were identified, and 193 target mRNAs including 103 for known and 90 for novel miRNAs were validated in one or more of tissues examined. The interesting finding was that over 50% of novel miRNAs were expressed exclusively in flowers or berries where they had tissue-specific cleavage roles on their target genes, especially, the breadth of their cleavage sites in flower tissues. Moreover, six novel miRNAs in berries were found to response to exogenous gibberellin (GA) and/or ethylene by real time RT-PCR (qRT-PCR) analysis, confirming their regulatory functions during berry development. Other finding was that about 93.6% of the known miRNAs possessed the high conservation in various tissues where their expression levels exhibited some dynamic variations during grapevine development. Significantly, it was found the phenomena that some Vv-miRNA families exist one key member that act as the main regulator of their target genes during grapevine development.
2021-08-24 | GSE182618 | GEO
Project description:Background:Many studies have indicated that the excessive use of computers (more than 3 hr/d) might be associated with an unhealthy life-style. Aim:The aim of this study was to evaluate the relationship between excessive computer use with the condition of the teeth and periodontium and the oral health behaviour of 18-year-olds. Design:Cross-sectional studies, using a questionnaire, were carried out on 1,611 18-year-olds from Poland. The questionnaire contained questions about socioeconomic status and information about health-related behaviour. The condition of their teeth and gingivae were clinically assessed. Results:Excessive (>3 h/d) computer use was reported by 492 (31%) of participants, who had an increased frequency of unfilled cavities (1.97 vs. 2.27, p = .047) and a higher risk of oral hygiene neglect (e.g., using dental floss 41% vs. 34%, p = .009). Excessive computer use was also seen to be associated with poor dietary habits. Individuals who declared excessive computer use also had a higher risk of gingival bleeding (35% vs. 29%, p = .009). Conclusion:In the group studied, excessive computer use by adolescents constituted a risk factor for neglect of oral hygiene, poor dietary choices, and failure to benefit from oral health care. Therefore, these aspects should be included in the risk assessment of oral disease and incorporated into educational programs that promote a healthy lifestyle.
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Project description:In total, 77 rhizobial strains isolated from the root nodules of T. repens, inhabiting heavy metal-contaminated waste heap (36 isolates) and control grassland (41 ones) in southern Poland, were analyzed for genome polymorphism and strength of the heavy metals' (mainly Zn, Pb, Cd) selective pressure on bacterial genome polymorphism using two PCR-based techniques, ERIC- (enterobacterial repetitive intergenic consensus) and REP-PCR (repetitive extragenic palindromic) sequences. Both methods of different discriminatory power index (D) (ERIC-PCR D?=?0.9737; REP-PCR D?=?0.9826) allowed to distinguish 47 and 44 rhizobial strains, respectively. Combined analysis of ERIC-PCR and REP-PCR DNA amplicons differentiated all tested isolates. Both ERIC- and REP-PCR DNA fingerprinting techniques showed significant decline of the genome polymorphism (h) in rhizobial population from metalliferous waste heap (h?=?0.89?±?0.03; h?=?0.90?±?0.02, respectively) compared to rhizobia from control non-metalliferous area (h?=?0.99?±?0.01; h?=?0.98?±?0.02, respectively) as well as substantial differences in the genomic polymorphism between both these populations (FST?=?0.162, p?=?0.008; FST?=?0.170, p?=?0.000, respectively).
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