Unknown

Dataset Information

0

Repression of the Hox gene abd-A by ELAV-mediated Transcriptional Interference.


ABSTRACT: Intergenic transcription is a common feature of eukaryotic genomes and performs important and diverse cellular functions. Here, we investigate the iab-8 ncRNA from the Drosophila Bithorax Complex and show that this RNA is able to repress the transcription of genes located at its 3' end by a sequence-independent, transcriptional interference mechanism. Although this RNA is expressed in the early epidermis and CNS, we find that its repressive activity is limited to the CNS, where, in wild-type embryos, it acts on the Hox gene, abd-A, located immediately downstream of it. The CNS specificity is achieved through a 3' extension of the transcript, mediated by the neuronal-specific, RNA-binding protein, ELAV. Loss of ELAV activity eliminates the 3' extension and results in the ectopic activation of abd-A. Thus, a tissue-specific change in the length of a ncRNA is used to generate a precise pattern of gene expression in a higher eukaryote.

SUBMITTER: Castro Alvarez JJ 

PROVIDER: S-EPMC8629391 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6158615 | biostudies-literature
| S-EPMC2705285 | biostudies-literature
| S-EPMC5940526 | biostudies-literature
| S-EPMC5302332 | biostudies-literature
| S-EPMC3441639 | biostudies-literature
| S-EPMC5001607 | biostudies-literature
| S-EPMC7017836 | biostudies-literature
| S-EPMC8513686 | biostudies-literature
| S-EPMC1461490 | biostudies-other
| S-EPMC4928683 | biostudies-literature