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Fluorescence immunoassay rapid detection of 2019-nCoV antibody based on the fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticle.

ABSTRACT: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has dramatically changed the world, is a highly contagious virus. The timely and accurate diagnosis of SARS-CoV-2 infections is vital for disease control and prevention. Here in this work, a fluorescence immunoassay was developed to detect 2019 Novel Coronavirus antibodies (2019-nCoV mAb). Fluorescent graphene quantum dots (GQDs) and Ag@Au nanoparticles (Ag@AuNPs) were successfully synthesized and characterized. Fluorescence resonance energy transfer (FRET) enables effective quenching of GQDs fluorescence by Ag@AuNPs. With the presence of 2019-nCoV mAb, a steric hindrance was observed between the Ag@AuNPs-NCP (2019-nCoV antigen) complex and GQDs, which reduced the FRET efficiency and restored the fluorescence of GQDs. The fluorescence enhancement efficiency has a satisfactory linear relationship with the logarithm of the 2019-nCoV mAb in a concentration range of 0.1 pg mL^-1-10 ng mL^-1, and the limit of detection was 50 fg mL^-1. The method has good selectivity. When the serum sample was spiked with 2019-nCoV mAb, the recovery rate was between 90.8% and 103.3%. The fluorescence immunosensor demonstrates the potential to complement the existing serological assays for COVID-19 diagnosis.

SUBMITTER: Li N

PROVIDER: S-EPMC8632743 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature

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Fluorescence immunoassay rapid detection of 2019-nCoV antibody based on the fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticle.

Li Nan N Shi Linhong L Zou Xue X Wang Tengfei T Wang Dongmei D Gong Zhengjun Z Fan Meikun M

Microchemical journal : devoted to the application of microtechniques in all branches of science 20211201

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has dramatically changed the world, is a highly contagious virus. The timely and accurate diagnosis of SARS-CoV-2 infections is vital for disease control and prevention. Here in this work, a fluorescence immunoassay was developed to detect 2019 Novel Coronavirus antibodies (2019-nCoV mAb). Fluorescent graphene quantum dots (GQDs) and Ag@Au nanoparticles (Ag@AuNPs) were successfully synthesized and characterized. Fluorescence res ...[more]

PMID: 34866656

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Project description:Methyl orange (MO) is an organic synthetic dye widely used in laboratory and industrial applications. In laboratory settings, it serves as an acid-base indicator due to its distinct color change in both acidic and alkaline environments. Industrially, it is primarily utilized in the textile industry for its ultraviolet (UV) absorption properties. However, the discharge and leakage of methyl orange into the environment can cause severe ecological damage and pose potential carcinogenic and teratogenic risks to human health. Therefore, detecting and quantifying the concentration of methyl orange in various matrices is crucial. This study reports the synthesis of graphene quantum dots (GQDs) from orange peel as a precursor, using ethanol and dimethylformamide (DMF) as solvents. Cyan (c-GQDs) and yellow (y-GQDs) graphene quantum dots were synthesized through a bottom-up hydrothermal method. The difference in color is attributed to the redshift caused by the varying ratio of pyridine nitrogen to pyrrole nitrogen. These GQDs exhibited notable optical properties, with c-GQDs emitting cyan fluorescence and y-GQDs emitting yellow fluorescence under UV light. To investigate fluorescence quenching effects, nine commonly used dyes were tested, and all were found to quench the fluorescence of y-GQDs, with methyl orange having the most significant effect. The fluorescence quenching of orange peel-derived GQDs in the presence of methyl orange is attributed to poor dispersion in DMF solution. Additionally, the GQDs possess high specific surface area, abundant surface functional groups, and excellent electronic conductivity, which contribute to their effective fluorescence quenching performance. The average thickness of y-GQDs (the vertical dimension from the substrate upwards) was 3.51 nm, confirming their graphene-like structure. They emitted yellow fluorescence within the wavelength range of 450-530 nm. Notably, a significant linear correlation was found between the concentration of methyl orange and the fluorescence intensity of y-GQDs (regression coefficient = 0.9954), indicating the potential of GQDs as effective sensing materials for organic pollutant detection.

Dataset Information

Fluorescence immunoassay rapid detection of 2019-nCoV antibody based on the fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticle.

Publications

Fluorescence immunoassay rapid detection of 2019-nCoV antibody based on the fluorescence resonance energy transfer between graphene quantum dots and Ag@Au nanoparticle.

Similar Datasets

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