PiggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition.
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ABSTRACT:
SUBMITTER: Wolff JH
PROVIDER: S-EPMC8633390 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Publications
piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition.
Wolff Jonas Holst JH Haldrup Jakob J Thomsen Emil Aagaard EA Andersen Sofie S Mikkelsen Jacob Giehm JG
Frontiers in genome editing 20211112
Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy. Here, we demonstrate piggyPrime, a transfected single-vector system based on piggyBac DNA transposition for genomic integration of all prime editing components in human cells allowing easy and effective transgenesis with prime editing efficacies up to 100% in cell lines. ...[more]
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