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Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging.

ABSTRACT: Super-resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3-fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state-of-the-art commercial laser scanning microscope systems.

SUBMITTER: Hennig S

PROVIDER: S-EPMC8634860 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging.

Hennig Simon S Manstein Dietmar J DJ

FEBS open bio 20210726 12

Super-resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3-fold increase in lateral and axial resolution compared to conventional confocal microscopes by combin ...[more]

PMID: 34228908

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