Live Imaging of Primary Neurons in Long-Term Cryopreserved Human Nerve Tissue.

ABSTRACT: Tissue cryopreservation provides a convenient solution for tackling one of the major problems in neuroscience research, namely, the scarce availability of human nerve tissues, especially if needed alive. While brain tissue can be used only postmortem, live nerve tissue can reasonably well be harvested from the periphery. A valuable source of primary neurons is the intestine, which compared with brain has the advantage to be safely accessible via endoscopy. The nerve tissue innervating the intestine (the enteric nervous system; ENS) can be sampled with regular endoscopic biopsy forceps and remains viable for multiple physiological and immunohistochemical tests, as previously demonstrated. Here, we present a method to preserve, over longer periods of time, human primary neurons contained in

SUBMITTER: Fortea M 

PROVIDER: S-EPMC8638677 | biostudies-literature | 2021 Nov-Dec

REPOSITORIES: biostudies-literature