Comparison of adherence tendencies of pesticide residues sprayed on small-, medium-, and large-sized tomatoes.
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ABSTRACT:
SUBMITTER: Nagata T
PROVIDER: S-EPMC8640702 | biostudies-literature |
REPOSITORIES: biostudies-literature
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Project description:BackgroundContamination of tomatoes by Salmonella can occur in agricultural settings. Little is currently understood about how agricultural inputs such as pesticide applications may impact epiphytic crop microflora and potentially play a role in contamination events. We examined the impact of two materials commonly used in Virginia tomato agriculture: acibenzolar-S-methyl (crop protectant) and copper oxychloride (pesticide) to identify the effects these materials may exert on baseline tomato microflora and on the incidence of three specific genera; Salmonella, Xanthomonas and Paenibacillus.ResultsApproximately 186 441 16S rRNA gene and 39 381 18S rRNA gene sequences per independent replicate were used to analyze the impact of the pesticide applications on tomato microflora. An average of 3 346 677 (634 892 974 bases) shotgun sequences per replicate were used for metagenomic analyses.ConclusionA significant decrease in the presence of Gammaproteobacteria was observed between controls and copper-treated plants, suggesting that copper is effective at suppressing growth of certain taxa in this class. A higher mean abundance of Salmonella and Paenibacillus in control samples compared to treatments may suggest that both systemic and copper applications diminish the presence of these genera in the phyllosphere; however, owing to the lack of statistical significance, this could also be due to other factors. The most distinctive separation of shared membership was observed in shotgun data between the two different sampling time-points (not between treatments), potentially supporting the hypothesis that environmental pressures may exert more selective pressures on epiphytic microflora than do certain agricultural management practices.
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Project description:Retrospective dietary exposure assessments were conducted for pesticides that have chronic effects on the thyroid and pesticides that have acute effects on the nervous system. Exposure assessments were performed using monitoring data collected by Member States under their official pesticide monitoring programmes in 2016, 2017 and 2018. Exposure estimates were obtained for 10 populations of consumers (i.e. from different countries and from different age groups) by means of a two-dimensional probabilistic model. Results were compared to those previously obtained for the years 2014, 2015 and 2016, and exposure did not change significantly over time. However, an increase of the sampling uncertainty was identified for one pesticide in a specific food commodity. Strategies are therefore recommended to reduce the sampling uncertainty and to anticipate potential problems before initiating a cumulative risk assessment.
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