Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts.
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ABSTRACT:
SUBMITTER: Remsik J
PROVIDER: S-EPMC8648257 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Characterization, isolation, and in vitro culture of leptomeningeal fibroblasts.
Remsik Jan J Saadeh Fadi F Tong Xinran X Li Min Jun MJ Snyder Jenna J Bale Tejus T Wu Jean J Derderian Camille C Guber David D Chi Yudan Y Murali Rajmohan R Boire Adrienne A
Journal of neuroimmunology 20210929
Meninges, or the membranous coverings of the brain and spinal cord, play host to dozens of morbid pathologies. In this study we provide a method to isolate the leptomeningeal cell layer, identify leptomeninges in histologic slides, and maintain leptomeningeal fibroblasts in in vitro culture. Using an array of transcriptomic, histological, and cytometric analyses, we identified ICAM1 and SLC38A2 as two novel markers of leptomeningeal cells in vivo and in vitro. Our results confirm the fibroblasto ...[more]
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