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CsKDO is a candidate gene regulating seed germination lethality in cucumber.

ABSTRACT: Seed germination plays an important role in the initial stage of plant growth. However, few related studies focused on lethality after seed germination in plants. In this study, we identified an Ethyl methanesulfonate (EMS) mutagenesis mutant Csleth with abnormal seed germination in cucumber (Cucumis sativus L.). The radicle of the Csleth mutant grew slowly and detached from the cotyledon until 14 d after sowing. Genetic analysis showed that the mutant phenotype of Csleth was controlled by a single recessive gene. MutMap+ and Kompetitive Allele Specific PCR (KASP) genotyping results demonstrated that Csa3G104930 encoding 3-deoxy-manno-octulosonate cytidylyltransferase (CsKDO) was the candidate gene of the Csleth mutant. The transition mutation of aspartate occurred in Csa3G104930 co-segregated with the phenotyping data. CsKDO was highly expressed in male flowers in wild type cucumbers. Subcellular localization results showed that CsKDO was located in the nucleus. Overall, these results suggest CsKDO regulates lethality during seed germination in cucumber.

SUBMITTER: Wang C

PROVIDER: S-EPMC8661486 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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CsKDO is a candidate gene regulating seed germination lethality in cucumber.

Wang Chen C Hao Ning N Xia Yutong Y Du Yalin Y Huang Ke K Wu Tao T

Breeding science 20210818 4

Seed germination plays an important role in the initial stage of plant growth. However, few related studies focused on lethality after seed germination in plants. In this study, we identified an Ethyl methanesulfonate (EMS) mutagenesis mutant Csleth with abnormal seed germination in cucumber (Cucumis sativus L.). The radicle of the Csleth mutant grew slowly and detached from the cotyledon until 14 d after sowing. Genetic analysis showed that the mutant phenotype of Csleth ...[more]

PMID: 34912168

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Project description:BackgroundExogenous melatonin plays a crucial role in various plant developmental processes and stress responses and has considerable potential for future agricultural applications. However, its effects on early cucumber seedling growth and resistance to alkaline stress have not been adequately explored. This study investigated the role of exogenous melatonin during the early growth stages of cucumber, specifically focusing on seed germination, post-germination seedling growth, and 1-leaf stage seedling growth, with particular emphasis on its influence on alkali stress resistance. These findings are intended to enhance the application of melatonin in cucumber seedling cultivation and provide a theoretical basis for promoting growth and improving stress tolerance in agricultural production.ResultsExogenous melatonin enhanced cucumber seed germination and early seedling growth with promoting and inhibitory effects at low and high concentrations, respectively. However, the effects of exogenous melatonin on cucumber growth varied at different developmental stages. Additionally, alkali stress significantly hampered the growth of cucumber seedlings; however, the external application of melatonin mitigated the damage caused by this stress. This protective effect was evidenced by a marked increase in the survival rate, stem diameter, and biomass of cucumber seedlings, along with a significant reduction in malondialdehyde content and electrolyte leakage rate. Further investigation revealed that exogenous melatonin promotes the accumulation of osmoregulatory substances, specifically soluble sugars, and proline, under alkaline stress. It also enhances the activities of antioxidant enzymes, including peroxidase, superoxide dismutase, catalase, and dehydroascorbate reductase, while significantly decreasing the accumulation of reactive oxygen species such as H2O2 and O2⋅-. Furthermore, exogenous melatonin increased the activities of PM-H+-ATPase and V-H+-ATPase and stimulated the expression of stress-related genes, thereby regulating Na+ and K+ homeostasis under alkali stress. Additionally, exogenous melatonin promoted the synthesis of endogenous melatonin in cucumbers subjected to alkaline stress by inducing the expression of melatonin synthase genes, namely, CsASMT, CsCOMT, CsTDC, and CsSNAT.ConclusionsExogenous melatonin promoted cucumber seed germination and seedling establishment and enhanced cucumber alkali stress tolerance by mediating osmotic adjustment, reactive oxygen species scavenging, ion homeostasis maintenance, endogenous melatonin synthesis, and expression of stress-related genes.

Project description:Seed enhancement technologies have the potential to improve germination and seedling growth under environmental stress. The effects of KIEM®, an innovative biostimulant based on lignin derivatives and containing plant-derived amino acids and molybdenum, were investigated on cucumber (Cucumis sativus L.) seed germination. To determine the metabolic targets of this product, biometric, transcriptional and biochemical analyses were carried out on both non-treated and KIEM®-treated seeds incubated for 24 and 48 h under standard (28°C) and heat stress (35°C) conditions. The application of the biostimulant as a seed treatment increased the percent germination (+6.54%) and fresh biomass (+13%) at 48 h, and decreased the content of H2O2 in treated seeds at 28°C (-70%) and at 35°C (-80%). These changes in biometric and biochemical properties were accompanied by changes in expression levels of the genes coding for ROS-producing (RBOH) and scavenging (SOD, CAT, GST) enzymes and their specific activity. In general, the treatment with KIEM® in heat-stress condition appeared to stimulate a higher accumulation of three scavenger gene transcripts: CuZnSOD (+1.78), MnSOD (+1.75), and CAT (+3.39), while the FeSOD isoform was dramatically downregulated (0.24). Moreover, the amount of non-protein thiols, important antioxidant molecules, was increased by the biostimulant after 48 h (+20%). Taken together these results suggest that KIEM® acts through mitigation of the effects of the oxidative stress. Moreover, after 48 h, the pre-sowing treatment with KIEM® increased the transcription levels (+1.5) and the activity of isocitrate lyase (+37%), a key enzyme of the glyoxylate cycle, suggesting a potential effect of this product in speeding up the germination process. Finally, the chemical characterization of KIEM® identified five essential and three non-essential amino acids, and others bioactive compounds, including five organic and inorganic acids that might be potentially involved in its activity. Based on these data, insights on the potential mechanism of action of the biostimulant, suggested that there are broader applications as a product able to increase seed tolerance to different abiotic stress typical of adverse environmental conditions.

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CsKDO is a candidate gene regulating seed germination lethality in cucumber.

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<i>CsKDO</i> is a candidate gene regulating seed germination lethality in cucumber.

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