Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns.
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ABSTRACT:
SUBMITTER: Huebinger J
PROVIDER: S-EPMC8664253 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Ultrarapid cryo-arrest of living cells on a microscope enables multiscale imaging of out-of-equilibrium molecular patterns.
Huebinger Jan J Grecco Hernan H Masip Martín E ME Christmann Jens J Fuhr Günter R GR Bastiaens Philippe I H PIH
Science advances 20211210 50
Imaging molecular patterns in cells by fluorescence micro- or nanoscopy has the potential to relate collective molecular behavior to cellular function. However, spatial and spectroscopic resolution is fundamentally limited by motional blur caused by finite photon fluxes and photobleaching. At physiological temperatures, photochemical reactivity does not only limit imaging at multiple scales but is also toxic to biochemical reactions that maintain cellular organization. Here, we present cryoprote ...[more]
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