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A far-red light-inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation.

ABSTRACT: [Figure: see text].

SUBMITTER: Wang X

PROVIDER: S-EPMC8664267 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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A far-red light-inducible CRISPR-Cas12a platform for remote-controlled genome editing and gene activation.

Wang Xinyi X Dong Kaili K Kong Deqiang D Zhou Yang Y Yin Jianli J Cai Fengfeng F Wang Meiyan M Ye Haifeng H

Science advances 20211210 50

The CRISPR-Cas12a has been harnessed as a powerful tool for manipulating targeted gene expression. The possibility to manipulate the activity of CRISPR-Cas12a with a more precise spatiotemporal resolution and deep tissue permeability will enable targeted genome engineering and deepen our understanding of the gene functions underlying complex cellular behaviors. However, currently available inducible CRISPR-Cas12a systems are limited by diffusion, cytotoxicity, and poor tissue permeability. Here, ...[more]

PMID: 34890237

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