Multiplex PCRs for identification of Escherichia coli virulence genes.
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ABSTRACT:
SUBMITTER: Pass MA
PROVIDER: S-EPMC86652 | biostudies-literature | 2000 May
REPOSITORIES: biostudies-literature
Publications
Multiplex PCRs for identification of Escherichia coli virulence genes.
Pass M A MA Odedra R R Batt R M RM
Journal of clinical microbiology 20000501 5
PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs. ...[more]
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