Generation of mixed murine organoids to model cellular interactions.
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ABSTRACT: Cell competition is a mechanism of interaction that dictates cell selection based on differences in cellular fitness. We designed a protocol to generate mixed murine organoids and enteroid monolayers used to study such complex cellular interactions in a mammalian system. This protocol is dedicated to follow the behavior of different cell populations over time, using (time-lapse) microscopy or transcriptome/proteome analysis. For complete details on the use and execution of this protocol, please refer to Krotenberg Garcia et al. (2021).
SUBMITTER: Krotenberg Garcia A
PROVIDER: S-EPMC8666359 | biostudies-literature |
REPOSITORIES: biostudies-literature
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