Profound changes in cerebrospinal fluid proteome and metabolic profile are associated with congenital hydrocephalus.

ABSTRACT: The aetiology of congenital hydrocephalus (cHC) has yet to be resolved. cHC manifests late in rodent gestation, and by 18-22 weeks in human fetuses, coinciding with the start of the major phase of cerebral cortex development. Previously we found that cerebrospinal fluid (CSF) accumulation is associated with compositional changes, folate metabolic impairment and consequential arrest in cortical development. Here, we report a proteomics study on hydrocephalic and normal rat CSF using LC-MSMS and a metabolic pathway analysis to determine the major changes in metabolic and signalling pathways. Non-targeted analysis revealed a proteome transformation across embryonic days 17-20, with the largest changes between day 19 and 20. This provides evidence for a physiological shift in CSF composition a

SUBMITTER: Requena-Jimenez A 

PROVIDER: S-EPMC8669293 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature