Induction of input-specific spine shrinkage on dendrites of rodent hippocampal CA1 neurons using two-photon glutamate uncaging.
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ABSTRACT:
SUBMITTER: Jang J
PROVIDER: S-EPMC8672044 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
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Induction of input-specific spine shrinkage on dendrites of rodent hippocampal CA1 neurons using two-photon glutamate uncaging.
Jang Jinyoung J Anisimova Margarita M Oh Won Chan WC Zito Karen K
STAR protocols 20211211 4
Shrinkage and loss of dendritic spines are vital components of the neuronal plasticity that supports learning. To investigate the mechanisms of spine shrinkage and loss, Oh and colleagues established a two-photon glutamate uncaging protocol that reliably induces input-specific spine shrinkage on dendrites of rodent hippocampal CA1 pyramidal neurons. Here, we provide a detailed description of that protocol and also an optimized version that can be used to induce input- and synapse-specific shrink ...[more]
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