Dataset Information

Decreased miR-940 expression can predict a negative prognosis in early-stage nonsmoking female lung adenocarcinoma.

ABSTRACT:

Background

Early-stage female lung adenocarcinoma is the most common type of lung cancer encountered in thoracic surgery departments. Tumor-node-metastasis (TNM) staging does not adequately explain a significant stratification phenomenon in the prognosis of patients with stage I lung adenocarcinoma. We aimed to investigate the contributory role of miR-940 in the prognosis prediction.

Methods

We analyzed the microRNA (miRNA) expression level in tumor tissues (high-risk group vs. low-risk group) from 12 non-smoking female patients with stage I lung adenocarcinoma using miRNA array. Bioinformatic analyses of miR-940 were also carried out based on the public database. Then, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) tests of the tissue samples were further validated. And miR-940's function was analyzed and potential target genes were predicted.

Results

In all, 24 miRNAs were found to be significantly different between the high-risk group and low-risk group. The expression level of miR-940 was lower in tumor tissue (P=0.011), and the survival rate in the high miR-940 group was higher [hazard ratio (HR) =0.688; P=0.011]. Gene Ontology (GO) analysis showed that the assembly functions of targets regulated by miR-940 were mainly enriched in regulation of myeloid cell differentiation, G1/S transition of mitotic cell cycle, and cellular response to environmental stimulus. miR-940 is involved in transforming growth factor-beta (TGF-beta) signaling pathway; TNF signaling pathway; and estrogen signaling pathway. The number of lung adenocarcinoma cells (A549) was significantly decreased after miR-940 was transfected. Ten epithelial-to-mesenchymal-transition (EMT)-associated genes (MMP9, ZEB1, CDH1, KRT8, KRT18 KET19, TWIST1, VIM, SNAI1, and SNAI2) were found to be significantly related to miR-940.

Conclusions

The present study showed that miR-940 might be a protective factor for positive prognosis in early stage nonsmoking female lung adenocarcinoma, with transforming growth factor-beta (TGF-beta) pathway, TNF pathway, and matrix metalloprotein (MMP9) being potential targets.

SUBMITTER: Ma Q

PROVIDER: S-EPMC8674601 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Decreased <i>miR-940</i> expression can predict a negative prognosis in early-stage nonsmoking female lung adenocarcinoma.

Ma Qianli Q Zhang Jin J Huang Jingjing J Wang Xiaowei X Xiao Fei F Xing Huajie H Wang Ye Y Guo Yongqing Y Shi Bin B Song Zhiyi Z Liu Deruo D Si Chaozeng C Horinouchi Hidehito H Liang Chaoyang C

Translational lung cancer research 20211101 11

<h4>Background</h4>Early-stage female lung adenocarcinoma is the most common type of lung cancer encountered in thoracic surgery departments. Tumor-node-metastasis (TNM) staging does not adequately explain a significant stratification phenomenon in the prognosis of patients with stage I lung adenocarcinoma. We aimed to investigate the contributory role of <i>miR-940</i> in the prognosis prediction.<h4>Methods</h4>We analyzed the microRNA (miRNA) expression level in tumor tissues (high-risk group ...[more]

PMID: 35004257

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