Unknown

Dataset Information

0

Gβγ regulates mitotic Golgi fragmentation and G2/M cell cycle progression.


ABSTRACT: Heterotrimeric G proteins (αβγ) function at the cytoplasmic surface of a cell's plasma membrane to transduce extracellular signals into cellular responses. However, numerous studies indicate that G proteins also play noncanonical roles at unique intracellular locations. Previous work has established that G protein βγ subunits (Gβγ) regulate a signaling pathway on the cytoplasmic surface of Golgi membranes that controls the exit of select protein cargo. Now, we demonstrate a novel role for Gβγ in regulating mitotic Golgi fragmentation, a key checkpoint of the cell cycle that occurs in the late G2 phase. We show that small interfering RNA-mediated depletion of Gβ1 and Gβ2 in synchronized cells causes a decrease in the number of cells with fragmented Golgi in late G2 and a delay of entry into mitosis and progression through G2/M. We also demonstrate that during G2/M Gβγ acts upstream of protein kinase D and regulates the phosphorylation of the Golgi structural protein GRASP55. Expression of Golgi-targeted GRK2ct, a Gβγ-sequestering protein used to inhibit Gβγ signaling, also causes a decrease in Golgi fragmentation and a delay in mitotic progression. These results highlight a novel role for Gβγ in regulation of Golgi structure.

SUBMITTER: Rajanala K 

PROVIDER: S-EPMC8684744 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC9925316 | biostudies-literature
| S-EPMC4614108 | biostudies-literature
| S-EPMC3123354 | biostudies-literature
| S-EPMC4216517 | biostudies-literature
| S-EPMC2063732 | biostudies-literature
| S-EPMC4268692 | biostudies-literature
| S-EPMC3564543 | biostudies-literature
| S-EPMC10871222 | biostudies-literature
| S-EPMC3680538 | biostudies-literature
| S-EPMC6760211 | biostudies-literature