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Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366.


ABSTRACT:

Background

Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, is male sterile under cold conditions (12 °C with 12 h of daylight) but fertile under normal temperature (20 °C with 12 h of daylight).

Results

During meiosis, BS366 was defective in forming tetrads and dyads due to the abnormal cell plate. During pollen development, unusual vacuolated pollen that could not accumulate starch grains at the binucleate stage was also observed. Transcriptome analysis revealed that genes involved in the meiotic process, such as sister chromatid segregation and microtubule-based movement, were repressed, while genes involved in DNA and histone methylation were induced in BS366 under cold conditions. MethylRAD was used for reduced DNA methylation sequencing of BS366 spikes under both cold and control conditions. The differentially methylated sites (DMSs) located in the gene region were mainly involved in carbohydrate and fatty acid metabolism, lipid metabolism, and transport. Differentially expressed and methylated genes were mainly involved in cell division.

Conclusions

These results indicated that the methylation of genes involved in carbon metabolism or fatty acid metabolism might contribute to male sterility in BS366 spikes, providing novel insight into the molecular mechanism of wheat male sterility.

SUBMITTER: Liu YJ 

PROVIDER: S-EPMC8686610 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Comparative transcriptome and DNA methylation analysis in temperature-sensitive genic male sterile wheat BS366.

Liu Yong-Jie YJ   Li Dan D   Gong Jie J   Wang Yong-Bo YB   Chen Zhao-Bo ZB   Pang Bin-Shuang BS   Chen Xian-Chao XC   Gao Jian-Gang JG   Yang Wei-Bing WB   Zhang Feng-Ting FT   Tang Yi-Miao YM   Zhao Chang-Ping CP   Gao Shi-Qing SQ  

BMC genomics 20211220 1


<h4>Background</h4>Known as the prerequisite component for the heterosis breeding system, the male sterile line determines the hybrid yield and seed purity. Therefore, a deep understanding of the mechanism and gene network that leads to male sterility is crucial. BS366, a temperature-sensitive genic male sterile (TGMS) line, is male sterile under cold conditions (12 °C with 12 h of daylight) but fertile under normal temperature (20 °C with 12 h of daylight).<h4>Results</h4>During meiosis, BS366  ...[more]

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