Browse
Submit Data
Databases
API
Help

Dataset Information

31 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Selective Detection of Penicillin G Antibiotic in Milk by Molecularly Imprinted Polymer-Based Plasmonic SPR Sensor.

ABSTRACT: Molecularly imprinted polymer-based surface plasmon resonance sensor prepared using silver nanoparticles was designed for the selective recognition of Penicillin G (PEN-G) antibiotic from both aqueous solution and milk sample. PEN-G imprinted sensors (NpMIPs) SPR sensor was fabricated using poly (2-hydroxyethyl methacrylate-N-methacroyl-(L)-cysteine methyl ester)-silver nanoparticles-N-methacryloyl-L-phenylalanine methyl ester polymer by embedding silver nanoparticles (AgNPs) into the polymeric film structure. In addition, a non-imprinted (NpNIPs) SPR sensor was prepared by utilizing the same polymerization recipe without addition of the PEN-G template molecule to evaluate the imprinting effect. FTIR-ATR spectrophotometer, ellipsometer, contact angle measurements were used for the characterization of NpMIPs SPR sensors. The linear concentration range of 0.01-10 ng/mL PEN-G was studied for kinetic analyses. The augmenting effect of AgNPs used to increase the surface plasmon resonance signal response was examined using polymer-based PEN-G imprinted (MIPs) sensor without the addition of AgNPs. The antibiotic amount present in milk chosen as a real sample was measured by spiking PEN-G into the milk. According to the Scatchard, Langmuir, Freundlich and Langmuir-Freundlich adsorption models, the interaction mechanism was estimated to be compatible with the Langmuir model.

SUBMITTER: Bakhshpour M

PROVIDER: S-EPMC8698653 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Similar Datasets

Electrochemical Sensor Based on Molecularly Imprinted Polymer for the Detection of Cefalexin.

Project description:In this study, a new electrochemical sensor was developed for the detection of cefalexin (CFX), based on the use of a molecularly imprinted polymer (MIP) obtained by electro?polymerization in an aqueous medium of indole-3-acetic acid (I3AA) on a glassy carbon electrode (GCE) and on boron-doped diamond electrode (BDDE). The two different electrodes were used in order to assess how their structural differences and the difference in the potential applied during electrogeneration of the MIP translate to the performances of the MIP sensor. The quantification of CFX was performed by using the electrochemical signal of a redox probe before and after the rebinding of the template. The modified electrode was characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The influence of different parameters on the fabrication of the sensor was tested, and the optimized method presented high selectivity and sensitivity. The MIP-based electrode presented a linear response for CFX concentration range of 10 to 1000 nM, and a limit of detection of 3.2 nM and 4.9 nM was obtained for the BDDE and the GCE, respectively. The activity of the sensor was successfully tested in the presence of some other cephalosporins and of other pharmaceutical compounds. The developed method was successfully applied to the detection of cefalexin from real environmental and pharmaceutical samples.

| S-EPMC6468396 | biostudies-literature

Novel Metal-Free Fluorescent Sensor Based on Molecularly Imprinted Polymer N-CDs@MIP for Highly Selective Detection of TNP.

Project description:In this article, we designed a fluorometric sensor based on nitrogen-passivated carbon dots infused with a molecularly imprinted polymer (N-CDs@MIP) via a reverse microemulsion technique using 3-aminopropyltriethoxysilane as a functional monomer, tetraethoxysilane as a cross-linker, and 2,4,6-trinitrophenol (TNP) as a template. The synthesized probe was used for selective and sensitive detection of trace amounts of TNP. The infusion of N-CDs (QY-21.6 percent) with a molecularly imprinted polymer can increase the fluorescent sensor sensitivity to detect TNP. Removal of template molecules leads to the formation of a molecularly imprinted layer, and N-CDs@MIP fluorescence response was quenched by TNP. The developed fluorescence probe shows a fine linear range from 0.5 to 2.5 nM with a detection limit of 0.15 nM. The synthesized fluorescent probe was used to analyze TNP in regular tap and lake water samples.

| S-EPMC8756778 | biostudies-literature

Novel fluorescence sensor for the selective recognition of tetracycline based on molecularly imprinted polymer-capped N-doped carbon dots.

Project description:A novel fluorescent probe based on molecularly imprinted polymers (MIPs) coupled with N-doped carbon dots (CDs) was prepared and used for specific recognition and sensitive determination of tetracycline (TC). N-doped CDs were synthesized using citric acid as a carbon source and ethylenediamine as a nitrogen source by a microwave assisted pyrolysis method. The determination conditions such as the solvents, material amount, pH value, and temperature were optimized. The CDs-MIPs have the best quenching on TC in water. The proposed method used for TC determination in milk powder samples had a detection limit of 0.054 μg mL-1 and a wide range of 0.5-30 μg mL-1. Meanwhile, satisfactory recoveries were obtained ranging from 95 to 108%. Oxytetracycline, chlorotetracycline and most of the coexisting substances showed no obvious interference indicating that the CDs-MIP probe exhibited high selectivity due to the presence of imprinted sites. Charge transfer from CDs-MIPs to TC may be through the mechanism of fluorescence quenching. This work gives a feasible strategy for the synthesis of N-doped carbon dot based molecularly imprinted polymers used as a fluorescent sensor in the food analysis field.

| S-EPMC9429051 | biostudies-literature

Molecularly Imprinted Magnetic Fluorescent Nanocomposite-Based Sensor for Selective Detection of Lysozyme.

Project description:A new strategy for the design and construction of molecularly imprinted magnetic fluorescent nanocomposite-based-sensor is proposed. This multifunctional nanocomposite exhibits the necessary optics, magnetism and biocompatibility for use in the selective fluorescence detection of lysozyme. The magnetic fluorescent nanocomposites are prepared by combining carboxyl- functionalized Fe3O4 magnetic nanoparticles with l-cysteine-modified zinc sulfide quantum dots (MNP/QDs). Surface molecular imprinting technology was employed to coat the lysozyme molecularly imprinted polymer (MIP) layer on the MNP/QDs to form a core-shell structure. The molecularly imprinted MNP/QDs (MNP/QD@MIPs) can rapidly separate the target protein and then use fluorescence sensing to detect the protein; this reduces the background interference, and the selectivity and sensitivity of the detection are improved. The molecularly imprinted MNP/QDs sensor presented good linearity over a lysozyme concentration range from 0.2 to 2.0 μM and a detection limit of 4.53 × 10-3 μM for lysozyme. The imprinting factor of the MNP/QD@MIPs was 4.12, and the selectivity coefficient ranged from 3.19 to 3.85. Furthermore, the MNP/QD@MIPs sensor was applied to detect of lysozyme in human urine and egg white samples with recoveries of 95.40-103.33%. Experimental results showed that the prepared MNP/QD@MIPs has potential for selective magnetic separation and fluorescence sensing of target proteins in biological samples.

| S-EPMC8232576 | biostudies-literature

Molecularly Imprinted Polymer Materials as Selective Recognition Sorbents for Explosives: A Review.

Project description:Explosives are of significant interest to homeland security departments and forensic investigations. Fast, sensitive and selective detection of these chemicals is of great concern for security purposes as well as for triage and decontamination in contaminated areas. To this end, selective sorbents with fast binding kinetics and high binding capacity, either in combination with a sensor transducer or a sampling/sample-preparation method, are required. Molecularly imprinted polymers (MIPs) show promise as cost-effective and rugged artificial selective sorbents, which have a wide variety of applications. This manuscript reviews the innovative strategies developed in 57 manuscripts (published from 2006 to 2019) to use MIP materials for explosives. To the best of our knowledge, there are currently no commercially available MIP-modified sensors or sample preparation methods for explosives in the market. We believe that this review provides information to give insight into the future prospects and potential commercialization of such materials. We warn the readers of the hazards of working with explosives.

| S-EPMC6572358 | biostudies-literature

Microfluidic Affinity Sensor Based on a Molecularly Imprinted Polymer for Ultrasensitive Detection of Chlorpyrifos.

Project description:The persistent use of pesticides in the agriculture field remains a serious issue related to public health. In the present work, molecularly imprinted polymer thin films were developed using electropolymerization of pyrrole (py) onto gold microelectrodes followed by electrodeposition for the selective detection of chlorpyrifos (CPF). The molecularly imprinted polymer (MIP) was synthesized by the electrochemical deposition method, which allowed in-line transfer of MIP on gold microelectrodes without using any additional adhering agents. Various parameters such as pH, monomer ratio, scan rate, and deposition cycle were optimized for sensor fabrication. The sensor was characterized at every stage of fabrication using various spectroscopic, microscopic, and electrochemical techniques. The sensor requires only 2 μL of the analyte and its linear detection range was found to be 1 μM to 1 fM. The developed sensor's limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.93 and 2.82 fM, respectively, with a sensitivity of 3.98 (μA/(μM)/ mm2. The sensor's shelf life was tested for 70 days. The applicability of the sensor in detecting CPF in fruit and vegetable samples was also assessed out with recovery % between 91 and 97% (RSD < 5%). The developed sensor possesses a huge commercial potential for on-field monitoring of pesticides.

| S-EPMC7745425 | biostudies-literature

Gut microbiota derived trimethylamine N-oxide (TMAO) detection through molecularly imprinted polymer based sensor.

Project description:Trimethylamine N-oxide (TMAO), a microbiota-derived metabolite has been implicated in human health and disease. Its early detection in body fluids has been presumed to be significant in understanding the pathogenesis and treatment of many diseases. Hence, the development of reliable and rapid technologies for TMAO detection may augment our understanding of pathogenesis and diagnosis of diseases that TMAO has implicated. The present work is the first report on the development of a molecularly imprinted polymer (MIP) based electrochemical sensor for sensitive and selective detection of TMAO in body fluids. The MIP developed was based on the polypyrrole (PPy), which was synthesized via chemical oxidation polymerization method, with and without the presence of TMAO. The MIP, NIP and the non-sonicated polymer (PPy-TMAO) were separately deposited electrophoretically onto the hydrolyzed indium tin oxide (ITO) coated glasses. The chemical, morphological, and electrochemical behavior of MIP, non-imprinted polymer (NIP), and PPy-TMAO were characterized using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and electrochemical techniques. The detection response was recorded using differential pulse voltammetry (DPV), which revealed a decrease in the peak current with the increase in concentration of TMAO. The MIP sensor showed a dynamic detection range of 1-15 ppm with a sensitivity of 2.47 µA mL ppm-1 cm-2. The developed sensor is easy to construct and operate and is also highly selective to detect TMAO in body fluids such as urine. The present research provides a basis for innovative strategies to develop sensors based on MIP to detect other metabolites derived from gut microbiota that are implicated in human health and diseases.

| S-EPMC7809026 | biostudies-literature

Molecularly imprinted polymer decorated nanoporous gold for highly selective and sensitive electrochemical sensors.

Project description:Electrochemical nanosensors based on nanoporous gold leaf (NPGL) and molecularly imprinted polymer (MIP) are developed for pharmaceutical analysis by using metronidazole (MNZ) as a model analyte. NPGL, serving as the loading platform for MIP immobilization, possesses large accessible surface area with superb electric conductivity, while electrochemically synthesized MIP thin layer affords selectivity for specific recognition of MNZ molecules. For MNZ determination, the hybrid electrode shows two dynamic linear range of 5 × 10(-11) to 1 × 10(-9) mol L(-1) and 1 × 10(-9) to 1.4 × 10(-6) mol L(-1) with a remarkably low detection limit of 1.8 × 10(-11) mol L(-1) (S/N = 3). In addition, the sensor exhibits high binding affinity and selectivity towards MNZ with excellent reproducibility and stability. Finally, the reliability of MIP-NPGL for MNZ detection is proved in real fish tissue samples, demonstrating the potential for the proposed electrochemical sensors in monitoring drug and biological samples.

| S-EPMC4287724 | biostudies-other

Molecularly imprinted polymer based electrochemical sensor for quantitative detection of SARS-CoV-2 spike protein.

Project description:The continued spread of the coronavirus disease and prevalence of the global pandemic is exacerbated by the increase in the number of asymptomatic individuals who unknowingly spread the SARS-CoV-2 virus. Although remarkable progress is being achieved at curtailing further rampage of the disease, there is still the demand for simple and rapid diagnostic tools for early detection of the COVID-19 infection and the following isolation. We report the fabrication of an electrochemical sensor based on a molecularly imprinted polymer synthetic receptor for the quantitative detection of SARS-CoV-2 spike protein subunit S1 (ncovS1), by harnessing the covalent interaction between 1,2-diols of the highly glycosylated protein and the boronic acid group of 3-aminophenylboronic acid (APBA). The sensor displays a satisfactory performance with a reaction time of 15 min and is capable of detecting ncovS1 both in phosphate buffered saline and patient's nasopharyngeal samples with LOD values of 15 fM and 64 fM, respectively. Moreover, the sensor is compatible with portable potentiostats thus allowing on-site measurements thereby holding a great potential as a point-of-care testing platform for rapid and early diagnosis of COVID-19 patients.

| S-EPMC8626155 | biostudies-literature

Bio-Inspired Molecularly Imprinted Polymer Electrochemical Sensor for Cortisol Detection Based on O-Phenylenediamine Optimization.

Project description:This paper presents a comprehensive investigation of the various parameters involved in the fabrication of a molecularly imprinted polymer (MIP) sensor for the detection of cortisol. Parameters such as monomer concentration, electropolymerization cycles, pH, monomer-template ratio, template removal technique, and rebinding time were optimized to establish a more consistent and effective method for the fabrication of MIP sensors. Under the optimized conditions, the MIP sensor demonstrated a proportional decrease in differential pulse voltammetry peak currents with increasing cortisol concentration in the range of 0.1 to 100 nM. The sensor exhibited excellent sensitivity, with a limit of detection of 0.036 nM. Selectivity experiments using a non-imprinted polymer sensor confirmed the specific binding affinity of the MIP sensor for cortisol, distinguishing it from other steroid hormones. This study provides crucial insights into the development of a reliable and sensitive strategy for cortisol detection using O-PD-based MIPs. These findings laid the foundation for further advancements in MIP research.

| S-EPMC10377510 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data