Dipolar Order Parameters in Large Systems With Fast Spinning.

ABSTRACT: Order parameters are a useful tool for quantifying amplitudes of molecular motions. Here we measure dipolar order parameters by recoupling heteronuclear dipole-dipole couplings under fast spinning. We apply symmetry based recoupling methods to samples spinning under magic angle at 60 kHz by employing a variable flip angle compound inversion pulse. We validate the methods by measuring site-specific ¹⁵N-¹H order parameters of a microcrystalline protein over a small temperature range and the same protein in a large, precipitated complex with antibody. The measurements of the order parameters in the complex are consistent with the observed protein undergoing overall motion within the assembly.

SUBMITTER: Franks WT 

PROVIDER: S-EPMC8699854 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature